Vasoactive intestinal peptide receptors in the airways of smokers with chronic bronchitis
Vasoactive intestinal peptide receptors in the airways of smokers with chronic bronchitis
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DOI：
10.1183/09031936.04.10031504
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发表时间：
2004-12-01
影响因子：
24.3
通讯作者：
Mapp, CE
中科院分区：
文献类型：
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作者：
Miotto, D;Boschetto, P;Mapp, CE
血管活性肠肽（Vasoactive intestinal peptide， VIP）是一种参与气道粘液分泌调节的神经肽。VIP的生物学功能是通过血管活性肠肽受体1型（VPAC1R）和2型（VPAC2R）两种受体介导的。本研究的目的是量化慢性支气管炎吸烟者中央气道中VPAC1R和VPAC2R的表达。手术标本取自33例因局部肺病变行开胸手术的吸烟者：23例有慢性支气管炎症状的吸烟者和10例肺功能正常的无症状吸烟者。通过免疫组化和显微镜分析，与无症状吸烟者相比，有慢性支气管炎症状的吸烟者的支气管上皮、支气管腺和血管中VPAC1R的表达增加，而VPAC2R的表达不增加。有慢性支气管炎症状的吸烟者支气管粘膜下层VPAC1R和VPAC2R阳性的单核细胞数量也有所增加。综上所述，慢性支气管炎吸烟者中央气道中1型和2型血管活性肠肽受体的表达增加，提示其可能参与慢性支气管炎的发病机制。
Vasoactive intestinal peptide (VIP) is a neuropeptide involved in the regulation of airway mucus secretion. The biological functions of VIP are mediated through two receptors, the vasoactive intestinal peptide receptor type 1 (VPAC1R) and type 2 (VPAC2R). The aim of this study was to quantify the expression of both VPAC1R and VPAC2R in the central airways of smokers with chronic bronchitis.Surgical specimens were obtained from 33 smokers undergoing thoracotomy for localised pulmonary lesions: 23 smokers with symptoms of chronic bronchitis and 10 asymptomatic smokers with normal lung function.By using immunohistochemical and microscopic analysis, an increased expression of VPAC1R, but not VPAC2R, was found in bronchial epithelium, bronchial glands and vessels of smokers with symptoms of chronic bronchitis compared with asymptomatic smokers. Smokers with symptoms of chronic bronchitis also had an increased number of mononuclear cells positive for both VPAC1R and VPAC2R in the bronchial submucosa.In conclusion, the expression of type 1 and type 2 vasoactive intestinal peptide receptors is increased in the central airways of smokers with chronic bronchitis, suggesting their possible involvement in the pathogenesis of chronic bronchitis.