LIF is more potent than BDNF In promoting neurite outgrowth of mammalian auditory neurons in vitro
LIF is more potent than BDNF In promoting neurite outgrowth of mammalian auditory neurons in vitro
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DOI：
10.1097/00001756-200102120-00019
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发表时间：
2001-02-12
期刊：
影响因子：
1.7
通讯作者：
Marzella, PL
中科院分区：
文献类型：
--
作者：
Gillespie, LN;Clark, GM;Marzella, PL
已知神经营养因子在Corti器官内听觉神经纤维的延伸和引导到其目标中起关键作用。耳聋后这些神经连接的维持将明显影响听力恢复治疗的疗效。生长因子白血病抑制因子（LIF），脑源性神经营养因子（BDNF）和转化生长因子-β 5（TGF-β 5）进行了测试，以促进神经突起生长的早期出生后大鼠听觉神经元的分离培养物的功效。我们的研究结果表明，虽然BDNF以强烈的方式增强神经突生长，但LIF更有效;此外，两种因子的联合给药具有更大的神经突生成能力。TGF-β 5虽然具有神经营养作用，但在培养的听觉神经元上没有促神经突活性。因此，LIF和BDNF可能是开发听力恢复药物疗法的潜在候选者。NeuroReport 12：275-279（C）2001 Lippincott威廉姆斯和威尔金斯。
Neurotrophic factors are known to play a crucial role in the elongation and guidance of auditory nerve fibres to their targets within the organ of Corti. Maintenance of these neural connections following deafness would clearly influence the efficacy of therapies for hearing recovery. The growth factors leukaemia inhibitory factor (LIF), brain-derived neurotrophic factor (BDNF) and transforming growth factor-beta 5 (TGF-beta5) were tested for their efficacy in promoting neurite outgrowth from dissociated cultures of early postnatal rat auditory neurons. Our results indicate that while BDNF enhances neurite outgrowth in a strong fashion, LIF is more potent; moreover, the combined administration of both factors has even greater neuritogenic capacities. TGF-beta5, although neurotrophic, has no neuritogenic activity on cultured auditory neurons. LIF and BDNF may therefore be potential candidates when developing pharmacological therapies for hearing recovery. NeuroReport 12:275-279 (C) 2001 Lippincott Williams & Wilkins.