Non-invasive blood glucose measurement by Fourier transform infrared spectroscopic analysis through the mucous membrane of the lip: application of a chalcogenide optical fiber system.
Non-invasive blood glucose measurement by Fourier transform infrared spectroscopic analysis through the mucous membrane of the lip: application of a chalcogenide optical fiber system.
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通过唇粘膜傅里叶变换红外光谱分析进行无创血糖测量：硫属化物光纤系统的应用。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
M. Shichiri
中科院分区：
文献类型：
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作者：
T. Uemura;K. Nishida;M. Sakakida;K. Ichinose;S. Shimoda;M. Shichiri
采用衰减全反射（ATR）棱镜，利用傅里叶变换红外（FT-IR）光谱技术，研究了通过嘴唇粘膜的无创血糖测量。为了实现ATR棱镜与唇粘膜的容易附着和容易控制ATR棱镜与唇粘膜的附着压力，使用了在尖端中内置有ATR棱镜的硫属化物光纤。在通过嘴唇粘膜的光谱中发现了与葡萄糖溶液相同的葡萄糖特异性峰，其波数为1080和1033 cm-1。ATR棱镜对唇粘膜的恒定压力为6.7 × 10（3）dyn/cm 2时，日内和日间测量的变异系数分别为3.8%和5.4%。为了消除基线漂移和除葡萄糖以外的身体成分的干扰，评价了餐后状态下测量的光谱与空腹状态下获得的背景光谱之间在1080 cm-1处的差值Δ λ作为个体特征。静脉脉冲注射葡萄糖后，1080 cm-1处的血糖浓度差值与血糖浓度变化有4 min的延迟，每日血糖监测中，血糖浓度差值与血糖浓度高于空腹水平的升高有高度显著的相关性（r = 0.920，P < 0.01）。从这些实验中，有人建议，FT-IR光谱与硫族化物光纤可以是有用的临床无创监测葡萄糖通过粘膜的嘴唇。
Non-invasive blood glucose measurement through the mucous membrane of the lip was investigated using Fourier transform infrared (FT-IR) spectroscopy with an attenuated total reflection (ATR) prism. To achieve easy attachment and easy control of attachment pressure of the ATR prism to the mucous membrane of the lip, a chalcogenide optical fiber with an ATR prism built in the tip was used. The same glucose-specific peaks at wave numbers of 1080 and 1033 cm-1 as glucose solutions were found in a spectrum through the mucous membrane of the lip. With a constant pressure of the ATR prism to the mucous membrane of the lip of 6.7 x 10(3) dyn/cm2, coefficients of variation of measurements within the day and of day-to-day measurements were 3.8 and 5.4% respectively. To eliminate baseline drifts and interference of body constituents other than glucose, the difference absorbances at 1080 cm-1 between spectra measured at the postprandial state and background spectrum obtained at the fasting state as an individual characteristic were evaluated. Following i.v. pulsatile injection of glucose, the difference absorbances at 1080 cm-1 nicely followed the changes in blood glucose concentrations with a time delay of 4 min. In daily blood glucose monitoring, a highly significant correlation between the difference absorbances and increases in blood glucose concentrations above the fasting level was obtained (r = 0.920, P < 0.01). From these experiments, it was suggested that FT-IR spectroscopy with a chalcogenide optical fiber could be useful clinically for non-invasive monitoring of glucose through the mucous membrane of the lip.