Comprehensive identification of MHC class II alleles in a cohort of Chinese rhesus macaques
Comprehensive identification of MHC class II alleles in a cohort of Chinese rhesus macaques
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中华恒河猴群体MHC II类等位基因的综合鉴定
DOI：
10.1007/s00251-014-0789-y
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发表时间：
2014-10-01
期刊：
影响因子：
3.2
通讯作者：
Ling, Fei
中科院分区：
文献类型：
--
作者：
Zhang, Huiling;Deng, Qing;Ling, Fei
恒河猴是研究人类各种疾病，特别是艾滋病和疫苗研究的重要动物模型。对这些疾病的易感性和/或抗性与主要组织相容性复合体（MHC）有关。为了深入了解中国恒河猴MHC背景，为进一步研究中国恒河猴MHC的实验应用提供依据，本研究通过基因克隆和测序的方法，对30只中国恒河猴Mamu-DPB 1、Mamu-DQB 1和Mamu-DR Balleles进行了研究。本研究共检测到66个等位基因，其中Mamu-DPB 1 14个，Mamu-DQB 1 20个，Mamu-DR Balleles 30个，Mamu-DPB 1 2个。有趣的是，其中一个高频率的Mamu-DPB 1等位基因在早期的研究中没有记录。其他11个等位基因，包括4个Mamu-DPB 1，3个Mamu-DQB 1和4个Mamu-DR Balleles也是新的。与MHC-DRB一样，在13只猴中检测到2个以上的Mamu-DPB 1序列，这表明它们可能代表基因重复。我们的数据还表明，中国恒河猴和以前报道的印度恒河猴的MHC II类等位基因的分布有相当多的差异。据我们所知，我们的结果全面揭示了MHC II等位基因的组合。这些信息不仅将促进对中国恒河猴MHC多态性的了解，而且将促进中国恒河猴在人类疾病研究中的应用。
Rhesus macaque is a very important animal model for various human diseases, especially for AIDS and vaccine research. The susceptibility and/or resistance to some of these diseases are related to the major histocompatibility complex (MHC). To gain insight into the MHC background and to facilitate the experimental use of Chinese rhesus macaques,Mamu-DPB1,Mamu-DQB1, andMamu-DRBalleles were investigated in 30 Chinese rhesus macaques through gene cloning and sequencing. A total of 66 alleles were identified in this study, including 14Mamu-DPB1, 20Mamu-DQB1, and 30Mamu-DRBalleles as well as 2 high-frequencyMamu-DPB1alleles. Interestingly, one of the high-frequencyMamu-DPB1alleles had been undocumented in earlier studies. Eleven of the other alleles, including fourMamu-DPB1, threeMamu-DQB1, and fourMamu-DRBalleles were also novel. Importantly, likeMHC-DRB, more than twoMamu-DPB1sequences per animal were detected in 13 monkeys, which suggested that they might represent gene duplication. Our data also indicated quite a few differences in the distribution of MHC class II alleles between the Chinese rhesus macaques and the previously reported Indian rhesus macaques. To our knowledge, our results revealed comprehensively the combination of MHC II alleles. This information will not only promote the understanding of Chinese rhesus macaque MHC polymorphism but will also facilitate the use of Chinese rhesus macaques in studies of human disease.