Uracil-DNA glycosylase activities in hyperthermophilic micro-organisms.
Uracil-DNA glycosylase activities in hyperthermophilic micro-organisms.
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DOI：
10.1111/j.1574-6968.1996.tb08491.x
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发表时间：
1996-10
影响因子：
2.1
通讯作者：
Athanasios Koulis;Don A. Cowan;Laurence H. Pearl;R. Savva
中科院分区：
文献类型：
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作者：
Athanasios Koulis;Don A. Cowan;Laurence H. Pearl;R. Savva
超嗜热菌存在于对它们的DNA的信息完整性造成增加的威胁的条件下，特别是通过水解损伤。在嗜温生物中，必须存在特定的活性来恢复和保护DNA的模板功能。在这项研究中，我们已经证明了存在的热稳定尿嘧啶-DNA糖基化酶的活动，在7个超嗜热菌;一个细菌：海栖热袍菌，和6个古菌：硫磺硫化叶菌，柴田硫化叶菌，酸热硫化叶菌，嗜热嗜热菌，激烈火球菌和Pyrobaculum islandicum。枯草芽孢杆菌噬菌体PBS 1的尿嘧啶-DNA糖基化酶抑制剂蛋白显示出对所有这些的活性，这表明超嗜热和嗜温尿嘧啶-DNA糖基化酶之间高度保守的三级结构。
Hyperthermophiles exist in conditions which present an increased threat to the informational integrity of their DNA, particularly by hydrolytic damage. As in mesophilic organisms, specific activities must exist to restore and protect this template function of DNA. In this study we have demonstrated the presence of thermally stable uracil-DNA glycosylase activities in seven hyperthermophiles; one bacterial: Thermotoga maritima, and six archaeal: Sulfolobus solfataricus, Sulfolobus shibatae, Sulfolobus acidocaldarius, Thermococcus litoralis, Pyrococcus furiosus and Pyrobaculum islandicum. Uracil-DNA glycosylase inhibitor protein of the Bacillus subtilis bacteriophage PBS1 shows activity against all of these, suggesting a highly conserved tertiary structure between hyperthermophilic and mesophilic uracil-DNA glycosylases.