Editing of an effector gene promoter sequence impacts plant-Phytophthora interaction
Editing of an effector gene promoter sequence impacts plant-Phytophthora interaction
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效应基因启动子序列的编辑影响植物与疫霉属的相互作用
DOI：
10.1111/jipb.12883
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发表时间：
2019-12-19
影响因子：
11.4
通讯作者：
Dong, Suomeng
中科院分区：
文献类型：
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作者：
Ochola, Sylvans;Huang, Jie;Dong, Suomeng
病原毒性（Avr）效应物与相应的植物抗性(R)蛋白相互作用，激活强大的植物免疫反应。虽然Avr基因的表达模式与它们的功能联系在一起已经很长时间了，但Avr基因表达模式如何影响植物与微生物的相互作用仍然不清楚。在这里，我们选择了PsAvr3b，它显示了大豆根病原菌疫霉的典型效应基因表达模式。为了调节基因表达，我们利用CRISPR/Cas9基因原位替换来自Actin（组成型表达）、PsXEG1（早期表达）和PsNLP1（后期表达）的启动子序列，设计了PsAvr3b启动子序列。不同启动子驱动的PsAvr3b在所有测试的感染时间点导致不同的表达水平。重要的是，那些PsAvr3b低表达的突变体成功地定殖了携带同源R基因Rps3b的大豆植株。为了分析植物对PsAvr3b表达水平的反应差异，我们在感染后24小时对不同感染样品进行了rna测序，发现了大豆免疫基因，包括一些以前未知的与抗性相关的基因。我们的研究强调了Avr基因表达的微调影响植物病害的亲和性，为病害防治管理中提高作物抗病性提供了线索。
Pathogen avirulence (Avr) effectors interplay with corresponding plant resistance (R) proteins and activate robust plant immune responses. Although the expression pattern of Avr genes has been tied to their functions for a long time, it is still not clear how Avr gene expression patterns impact plant-microbe interactions. Here, we selected PsAvr3b, which shows a typical effector gene expression pattern from a soybean root pathogen Phytophthora sojae. To modulate gene expression, we engineered PsAvr3b promoter sequences by in situ substitution with promoter sequences from Actin (constitutive expression), PsXEG1 (early expression), and PsNLP1 (later expression) using the CRISPR/Cas9. PsAvr3b driven by different promoters resulted in distinct expression levels across all the tested infection time points. Importantly, those mutants with low PsAvr3b expression successfully colonized soybean plants carrying the cognate R gene Rps3b. To dissect the difference in plant responses to the PsAvr3b expression level, we conducted RNA-sequencing of different infection samples at 24 h postinfection and found soybean immune genes, including a few previously unknown genes that are associated with resistance. Our study highlights that fine-tuning in Avr gene expression impacts the compatibility of plant disease and provides clues to improve crop resistance in disease control management.