RNA interference of cadherin gene expression in Spodoptera exigua reveals its significance as a specific Bt target
RNA interference of cadherin gene expression in Spodoptera exigua reveals its significance as a specific Bt target
复制标题
DOI：
10.1016/j.jip.2013.09.006
复制
发表时间：
2013-11-01
影响因子：
3.4
通讯作者：
Kim, Yonggyun
中科院分区：
文献类型：
--
作者：
Park, Youngjin;Kim, Yonggyun
钙粘蛋白是一种钙依赖性的细胞表面分子，被认为是苏云金芽孢杆菌Cry蛋白的靶分子。基于从454份五龄夜蛾中肠焦磷酸测序转录本中获得的部分钙粘蛋白基因，克隆了一个完整的钙粘蛋白开放阅读框（SeCad1）。其预测的氨基酸序列编码细胞外9个钙粘蛋白重复序列，跨膜和细胞内结构域。RT-PCR结果显示，SeCad1基因在发育的各个阶段均有表达，并在肠道组织中表达。在5龄时注射和喂养其特异性双链RNA（dsRNA(SeCad1)）均可抑制SeCad1基因的表达。抑制SeCad1的表达对小夜蛾蛹和成虫发育无显著影响。而dsRNA(seCad1) （100 ng/株）对苏云金芽孢杆菌的敏感性显著降低。aizawai。与此相反，dsRNA（seCad1）处理的幼虫对苏云金弧菌的敏感性没有变化。kurstaki。这些结果表明，SeCad1对苏云金芽孢杆菌Cry1C毒素具有特异性靶分子作用，而对Cry1A毒素没有特异性靶分子作用。(C) 2013爱思唯尔公司版权所有。
Cadherin is a calcium-dependent cell surface molecule and has been proposed to be a target molecule of Bacillus thuringiensis Cry proteins. Based on a partial cadherin gene obtained from 454 pyrosequencing transcripts of the fifth instar Spodoptera exigua midgut, a full open reading frame of cadherin gene (SeCad1) was cloned. Its predicted amino acid sequence encodes extracellular 9 cadherin repeats, a transmembrane, and intracellular domain. SeCad1 gene was expressed in all developmental stages specifically in gut tissue by RT-PCR analysis. Expression of SeCad1 gene was suppressed by both injection and feeding of its specific double-stranded RNA (dsRNA(seCad1)) in the fifth instar. The suppression of SeCad1 expression did not significantly influence on pupal and adult developments of S. exigua. However, the larvae treated with dsRNA(seCad1) (100 ng/larva) significantly reduced susceptibility to B. thuringiensis ssp. aizawai. In contrast, the dsRNA(seCad1)-treated larvae did not show any change in susceptibility to B. thuringiensis ssp. kurstaki. These results suggest that SeCad1 is a specific target molecule to Cry1C toxin from B. thuringiensis in S. exigua, but not to Cry1A toxin. (C) 2013 Elsevier Inc. All rights reserved.