Microfabricated capillary electrophoresis sensor for uranium (VI)
Microfabricated capillary electrophoresis sensor for uranium (VI)
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DOI：
10.1016/s0003-2670(01)00903-5
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发表时间：
2001-06-12
影响因子：
6.2
通讯作者：
Lu, Q
中科院分区：
文献类型：
--
作者：
Collins, GE;Lu, Q
砷酸盐III是一种金属变色配体，对镧系金属离子的络合反应非常敏感，将其应用于毛细管电泳微芯片，用于铀(VI)和各种稀土金属离子的检测。玻璃微芯片包含100微米深、200微米宽的微通道，以简单的十字形图案蚀刻，分离通道长度为80 mm，注入通道长度为8 mm。使用红色发光二极管(LED)光源和光电二极管阵列检测器实现了对阿森酸盐III金属络合物的检测。在背景电解液中加入CarBowax 20M，以消除电渗流并防止染料吸附在微通道壁上。用不到2分钟的时间实现了铀与4种稀土金属离子的分离。在本底电解液中加入二乙烯三胺五乙酸(DTPA)是消除稀土、过渡金属和碱土金属离子干扰的有效方法。将预络合的金属离子混合物直接负载注入到微通道中，在55岁以下的S中，当存在7个稀土杂质(每个杂质1.5ppm)时，检测下限为23 ppb。(C)2001年爱思唯尔科学公司保留所有权利。
Arsenate III, a metallochromic ligand colorimetrically sensitive to the metal complexation of lanthanide and actinide metal ions, is applied to a capillary electrophoresis microchip for the detection of uranium (VI) and various lanthanide metal ions. The glass microchip contained 100 mum deep by 200 mum wide microchannels etched in a simple cross pattern with an 80 mm separation channel length and an 8 mm injection channel length. Detection of the Arsenate III metal complexes is achieved using a red light emitting diode (LED) light source and a photodiode array detector. Carbowax 20M is incorporated into the background electrolyte in order to eliminate the electroosmotic flow and prevent dye adsorption on the microchannel walls. Separation of uranium from four lanthanide metal ions is demonstrated in under 2 min. The addition of diethylenetriaminepentaacetic acid (DTPA) to the background electrolyte is found to be an effective means of eliminating any interference from lanthanide, transition and alkaline earth metal ions. Direct load injection of a pre-complexed metal ion mixture onto the microchannel gave a detection limit of 23 ppb uranium (VI) in the presence of seven lanthanide impurities (1.5 ppm each) in under 55 s. (C) 2001 Elsevier Science B.V. All rights reserved.