Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures.
Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures.
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Sysmex UF-100 尿液流式细胞仪与超活染色样本和传统细菌培养物室计数的评估。
DOI：
10.1093/ajcp/112.1.25
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发表时间：
1999
影响因子：
3.5
通讯作者：
R. Rowan
中科院分区：
文献类型：
--
作者：
T. Kouri;Ulla Kähkönen;K. Malminiemi;R. Vuento;R. Rowan
我们使用 269 个未离心的尿液样本评估了 Sysmex UF-100 尿液流式细胞仪（TOA Medical Electronics，神户，日本），将其与使用明场和相差显微镜（参考方法）在 1 微升一次性室中进行的 Sternheimer 染色和颗粒计数进行比较。还提供了常规试纸分析、沉积物显微镜检查（182 个样本）和细菌培养（204 个样本）的结果。与手动室计数相比，使用 UF-100 检测尿 WBC 和 RBC 的可靠性非常高（分别为 r = 0.98 和 0.88）。细菌的鉴定与未离心标本的目视显微镜鉴定相同；与每毫升 > 10(3) 菌落形成单位的细菌培养物相比，敏感性为 55%，特异性为 90%。由于肾小管细胞和管型的计数较低，即使采用手动方法也很难评估肾损伤；与未离心室方法相比，采用标准手动 Sternheimer 染色沉积物分析，灵敏度为 65% 至 69%，特异性为 66% 至 91%，截止值分别为每微升 3 和 10 个颗粒。与室计数相比，UF-100 证明了肾损伤，其敏感性为 26% 至 69%，特异性为 92% 至 94%。使用 UF-100 尿液流式细胞仪进行自动尿液分析可节省大量时间和劳动力。当需要高灵敏度时，应进行目视显微镜检查以检测肾脏疾病。
We evaluated the Sysmex UF-100 urine flow cytometer (TOA Medical Electronics, Kobe, Japan) with 269 uncentrifuged urine specimens by comparing it with Sternheimer staining and particle counting in 1-microL disposable chambers with both brightfield and phase-contrast microscopy (the reference method). Results of routine test strip analysis, sediment microscopy (182 specimens), and bacterial culture (204 specimens) were also available. Detection of urinary WBCs and RBCs was highly reliable with the UF-100 compared with manual chamber counting (r = .98 and .88, respectively). Identification of bacteria was equal to that with visual microscopy of uncentrifuged specimens; sensitivity was 55%, and specificity 90%, compared with bacterial cultures at a cutoff of > 10(3) colony-forming units per milliliter. Renal damage was difficult to evaluate even with manual methods because of the low counts of renal tubular cells and casts; with standard manual Sternheimer-stained sediment analysis, sensitivity was 65% to 69% and specificity 66% to 91%, compared with the uncentrifuged chamber method at a cutoff of 3 and 10 particles per microliter, respectively. Renal damage was demonstrated with the UF-100 with a sensitivity of 26% to 69% and specificity 92% to 94%, compared with chamber counts. Automated urinalysis with the UF-100 urine flow cytometer offers considerable savings in time and labor. When high sensitivity is needed, visual microscopic review should be performed to detect renal disease.