Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations.
Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations.
复制标题
新的水凝胶系统消除了继代培养，并改善了非衰老间充质干细胞群体的保留率。
DOI：
10.2217/rme-2022-0140
复制
发表时间：
2023-01
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
比较可扩张组织模拟3D系统中的间充质干细胞/基质细胞（MSC）与传统2D系统中的体外扩张的生理行为。脂肪来源的MSC（ASC）在2D培养塑料或消除继代培养的3D水凝胶系统中连续培养6周。评估ASC的衰老、“干细胞样”MSC标记物以及其分泌组增加次级细胞群的能力。3D水凝胶系统导致更多再生的、非衰老的ASC群体的增强保留，这些ASC群体表现出“干细胞样”MSC表面标志物的表达增加。这项研究介绍了一种新型模块化3D系统的概念验证设计，该系统可以改善干细胞样细胞群的体外扩增，用于未来的再生治疗。新型组织模拟3D水凝胶系统增强了非衰老ASC群体在体外培养中长达6周的保留，并消除了传代培养的需要，提高了ASC及其分泌的生物制剂的再生能力。
To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system. Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population. The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers. This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies. Novel tissue-mimetic 3D hydrogel system enhances the retention of nonsenescent ASC populations in vitro for up to 6 weeks in culture and eliminates the need to subculture, improving regenerative capacity of ASCs and their secreted biologics.
登录
查看更多内容
影响因子：
3.7
作者：
Chowdhury F;Li Y;Poh YC;Yokohama-Tamaki T;Wang N;Tanaka TS
通讯作者：
Tanaka TS
DOI：
10.1146/annurev-pathol-121808-102144
发表时间：
2010
期刊：
Annual review of pathology
影响因子：
--
作者：
Coppé JP;Desprez PY;Krtolica A;Campisi J
通讯作者：
Campisi J
影响因子：
4.6
作者：
Gerardo, Heloisa;Lima, Ana;Graos, Mario
通讯作者：
Graos, Mario
影响因子：
4
作者：
Chen, Jingming;Chin, Adam;Taboas, Juan M.
通讯作者：
Taboas, Juan M.
影响因子：
9.7
作者：
Burnsed, Olivia A.;Schwartz, Zvi;Boyan, Barbara D.
通讯作者：
Boyan, Barbara D.