Functional analysis of the Enterococcus faecalis plasmid pAD1-encoded stability determinant par.
Functional analysis of the Enterococcus faecalis plasmid pAD1-encoded stability determinant par.
复制标题
粪肠球菌质粒 pAD1 编码的稳定性决定子 par 的功能分析。
DOI：
10.1111/j.1365-2958.1996.tb02488.x
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发表时间：
1996
影响因子：
3.6
通讯作者：
Sriram,SI
中科院分区：
文献类型：
--
作者：
Weaver,KE;Jensen,KD;Colwell,A;Sriram,SI
研究了pAD 1复制子编码的parstability determinant的分子结构和功能特征，其亲代编码两种会聚转录的RNA，分别命名为RNA I和RNA II，分别为210和65个核苷酸。RNA II的序列在很大程度上与RNA I互补，这表明RNA II可以作为反义RNA调节RNA I的功能。功能研究的结果与分离后杀伤系统的作用一致，这是第一个在革兰氏阳性菌中发现的，RNA I编码毒素，RNA II是解毒剂。这些成果包括：（i）在同一细胞中存在第二个完整的RNA II编码序列时，含有par的复制子不稳定;（ii）以宿主细胞生长速率为代价，依赖于par的高度不稳定载体的稳定;（iii）保护细胞免受RNA II反式表达过量RNA I的毒性作用。
The molecular organization and functional characteristics of the pAD1 replicon‐encodedparstability determinant were examined,parencodes two convergently transcribed RNAs of 210 and 65 nucleotides designated RNA I and RNA II, respectively. The sequence of RNA II is largely complementary to RNA I, suggesting that RNA II could regulate RNA I function as an anti‐sense RNA. Results of functional studies are consistent with a role forparas a post‐segregational killing system, the first to be identified in Gram‐positive bacteria, with RNA I encoding the toxin and RNA II the antidote. These results include: (i) destabilization ofpar‐containing replicons in the presence of a second completeparor the RNA II coding sequence in the same cell; (ii)par‐dependent stabilization of a highly unstable vector at the expense of host‐cell growth rate; and (iii) protection of cells from the toxic effects of overexpression of RNA I by RNA II suppliedin trans.