Obesity is induced in mice heterozygous for cyclooxygenase-2
Obesity is induced in mice heterozygous for cyclooxygenase-2
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DOI：
10.1016/s0090-6980(01)00136-8
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发表时间：
2001-07-01
影响因子：
2.9
通讯作者：
Bahouth, SW
中科院分区：
文献类型：
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作者：
Fain, JN;Ballou, LR;Bahouth, SW
在COX-2基因杂合子(COX-2(+/-))小鼠中，与纯合子COX-2(-/-)小鼠相比，体重增加33%。COX-2(+/-)小鼠的性腺脂肪垫重量是COX-2(-/-)小鼠的3.5~4.7倍，是野生型对照(+/+)小鼠的1.5~3.5倍。COX-2(+/-)小鼠的血清瘦素水平和培养脂肪组织的瘦素释放均高于对照组或COX-2(-/-)小鼠。COX-2(-/-)小鼠脂肪组织孵育24小时后，每个脂肪垫上PGE或6-酮-PGF(1α)的基础释放量分别减少了80%和95%。NS-398，一种特异性的COX-2抑制剂。抑制COX-1(-/-)小鼠脂肪组织中瘦素释放27%，COX-1(-/-)小鼠脂肪组织中31%，COX-2(+/-)小鼠脂肪组织中23%，而对COX-2(-/-)小鼠脂肪组织中瘦素释放无影响。这些数据表明，COX-2杂合子小鼠发生肥胖并不是由于脂肪组织释放瘦素的缺陷所致。(C)2001 Elsevier Science Inc.保留所有权利。
In mice heterozygous for the cyclooxygenase-2 gene (COX-2(+/-)) the body weight was enhanced by 33% as compared to homozygous COX-2(-/-) mice. The weights of the gonadal fat pads in COX-2(+/-) mice were enhanced by 3.5 to 4.7 fold as compared to COX-2(-/-) mice and by 1.5 to 3.5 fold as compared to wild-type controls(+/+) Serum leptin levels and leptin release by cultured adipose tissue of COX-2(+/-) mice were both elevated as compared to either control or COX-2(-/-) animals. The basal release of PGE, or 6 keto PGF(1 alpha) per fat pad over a 24 h incubation of adipose tissue was reduced by 80% and 95% respectively in tissue from COX-2(-/-) mice. NS-398, a specific COX-2 inhibitor. inhibited leptin release by 27% in adipose tissue from control mice, 31% in tissue from COX-1(-/-) mice and by 23% in tissue from COX-2(+/-) mice while having no effect on leptin release by adipose tissue from COX-2(-/-) mice. These data indicate that heterozygous COX-2 mice develop obesity which is not secondary to a defect in leptin release by adipose tissue. (C) 2001 Elsevier Science Inc. All rights reserved.