Evidence for retroviral intramolecular recombinations.
Evidence for retroviral intramolecular recombinations.
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逆转录病毒分子内重组的证据。
DOI：
10.1128/jvi.75.14.6348-6358.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Ma,Y
中科院分区：
文献类型：
--
作者：
Zhang,J;Ma,Y
由于是二倍体，逆转录病毒具有很高的重组率。天然存在的逆转录病毒在其 RNA 基因组的两端含有两个重复序列（R 区），并且这两个 R 区之间的重组发生率很高。我们推断重组可能发生在同一 RNA 分子内的两个序列之间（分子内）以及两个单独的 RNA 分子内存在的序列之间（分子间）。分子内重组通常会导致子代原病毒内的缺失。在这份报告中，我们证明了两个相同序列之间的分子内重组发生在嵌合 RNA 载体内。此外，同一RNA分子内两个相同序列之间的高重组率主要是由分子内重组造成的。
As a consequence of being diploid, retroviruses have a high recombination rate. Naturally occurring retroviruses contain two repeat sequences (R regions) flanking either end of their RNA genomes, and recombination between these two R regions occurs at a high rate. We deduced that recombination may occur between two sequences within the same RNA molecule (intramolecular) as well as between sequences present within two separate RNA molecules (intermolecular). Intramolecular recombination would usually result in a deletion within the progeny provirus. In this report, we demonstrate that intramolecular recombination between two identical sequences occurred within a chimeric RNA vector. In addition, high rates of recombination between two identical sequences within the same RNA molecule resulted mostly from intramolecular recombination.