The genomic organization and transcription of the ubiquitin genes of Trypanosoma cruzi.
The genomic organization and transcription of the ubiquitin genes of Trypanosoma cruzi.
复制标题
DOI：
10.1002/j.1460-2075.1988.tb02921.x
复制
发表时间：
1988-04
期刊：
影响因子：
--
通讯作者：
John Swindle;Janet;'. Ajioka;Harvey;'. Eisen;Bishnu Sanwal;Catherine Jacquemot;Zoe Browder;Gregory Buck
中科院分区：
文献类型：
--
作者：
John Swindle;Janet;'. Ajioka;Harvey;'. Eisen;Bishnu Sanwal;Catherine Jacquemot;Zoe Browder;Gregory Buck
我们在这里描述的组织泛素基因的寄生原生动物克氏锥虫。T. Cruzi含有超过100个遍在蛋白编码序列，所有这些序列都聚集在基因组的27 kb片段中。发现了两种类型的泛素编码序列。有五种融合基因（FUS 1 - 5）由泛素编码序列与基本的非泛素序列融合组成。霸王cruzi泛素融合蛋白与酿酒酵母UBI基因产物的同源性为84%。两种蛋白质的非泛素结构域有67%的同源性。存在五个多聚泛素编码基因（PUB），每个多聚泛素编码基因由不同长度的多聚泛素编码序列组成，并以较大融合基因的单拷贝终止。泛素基因的转录导致六种主要poly（A）+mRNA的产生。转录模式准确地反映了基因组组织，因为转录物由泛素融合编码序列的单个拷贝或不同长度的多聚泛素（最多52个泛素编码单元拷贝）组成，每个多聚泛素以泛素融合序列的单个拷贝结束。最后，PUB基因的5′端存在热激元件，并且转录模式在胁迫条件下发生改变。
We describe here the organization of the ubiquitin genes of the parasitic protozoan Trypanosoma cruzi. T. cruzi contains greater than 100 ubiquitin coding sequences all of which are clustered into a 27 kb segment of the genome. Two types of ubiquitin coding sequences were found. There are five fusion genes (FUS1‐5) consisting of a ubiquitin coding sequence fused to a basic non‐ubiquitin sequence. The T. cruzi ubiquitin fusion protein is 84% homologous to the product of the UBI gene of Saccharomyces cerevisiae. The non‐ubiquitin domains of the two proteins are 67% homologous. There are five polyubiquitin coding genes (PUB) each consisting of varying lengths of polyubiquitin coding sequence and terminating with a single copy of the larger fusion gene. Transcription of the ubiquitin genes results in the generation of six major poly(A)+ mRNAs. The pattern of transcription accurately reflects the genomic organization, in that the transcripts consist of either a single copy of the ubiquitin fusion coding sequence or varying lengths of polyubiquitin (up to 52 copies of the ubiquitin coding unit) each ending with a single copy of the ubiquitin fusion sequence. Finally, there are heat shock elements 5′ to the PUB genes and transcription patterns are altered under conditions of stress.