Insights into the role of earthworms on the optimization of microbial community structure during vermicomposting of sewage sludge by PLFA analysis.
Insights into the role of earthworms on the optimization of microbial community structure during vermicomposting of sewage sludge by PLFA analysis.
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DOI：
10.1016/j.wasman.2018.08.041
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发表时间：
2018-08
期刊：
影响因子：
8.1
通讯作者：
C. Zhao;Yong Wang;Yue Wang;Feijie Wu;Jiageng Zhang;Ruiyi Cui;Liguo Wang;H. Mu
中科院分区：
文献类型：
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作者：
C. Zhao;Yong Wang;Yue Wang;Feijie Wu;Jiageng Zhang;Ruiyi Cui;Liguo Wang;H. Mu
在本研究中，研究了蚯蚓对蚯蚓堆肥（VPs， with蚯蚓）中微生物群落结构和代谢功能的影响，以稳定多余污泥。比较了VPs和普通堆肥（不加蚯蚓）中PLFA剖面的动态变化。随着时间的推移，VPs的Shannon指数呈上升趋势，而CPs的Shannon指数呈下降趋势，说明蚯蚓活动增强了微生物群落多样性。与cp相比，vp组真菌和原生动物生物量显著增加。进一步的主成分分析（PCA）表明，蚯蚓对某些含有18:1ω9c、18:3ω3、18:3ω6、20:1ω9、20:2ω6和20:3ω6生物标志物的微生物有益。此外，VPs中单不饱和PLFAs与支链PLFAs的比例大于CPs，表明蚯蚓的穴居行为促进了通气条件，从而促进了原生动物等需氧微生物的生长和繁殖。这些结果表明，蚯蚓的活动导致了蚯蚓堆肥对剩余污泥稳定的总体优化。
In this study, the influences of earthworms on the structure of microbial community as well as the metabolic function in vermicomposting (VPs, with earthworms) for excess sludge stabilization were investigated. Comparison between the dynamic variation of PLFA profiles in VPs and common composting (CPs, with no earthworms) was conducted. The Shannon index was increased in VPs, while it was decreased in CPs with time, indicating earthworm activity enhanced microbial community diversity. The fungal and protozoal biomasses were significantly increased in VPs compared with CPs. Further researches by principal component analysis (PCA) indicated that earthworms benefited certain microorganisms containing biomarkers of 18:1ω9c, 18:3ω3, 18:3ω6, 20:1ω9, 20:2ω6 and 20:3ω6. Moreover, the ratios of monounsaturated to branched PLFAs in VPs were larger than those in CPs, suggesting the aeration condition was promoted by the burrowing behaviors of earthworms and therefore facilitated the growth and propagation of aerobic microorganisms, such as protozoa. Those results indicated that earthworm activity led to the general optimization of vermicomposting for excess sludge stabilization.