Postnatal neurogenesis and gliogenesis in the olfactory bulb from NG2-expressing progenitors of the subventricular zone
Postnatal neurogenesis and gliogenesis in the olfactory bulb from NG2-expressing progenitors of the subventricular zone
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DOI：
10.1523/jneurosci.3572-04.2004
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发表时间：
2004-11-17
影响因子：
5.3
通讯作者：
Gallo, V
中科院分区：
文献类型：
--
作者：
Aguirre, A;Gallo, V
我们使用2 '，3'-环核苷酸3 '-磷酸二酯酶（CNP）增强的绿色荧光蛋白（EGFP）转基因小鼠研究出生后脑室下区（SVZ）祖细胞的命运，重点是嗅球（OB）。CNP-EGFP小鼠出生后的OB含有EGFP（+）中间神经元和少突胶质细胞。在前SVZ中，大多数EGFP（+）祖细胞为NG 2（+）。这些NG 2（+）/EGFP（+）祖细胞表达OB中间神经元标记Er 81、成神经细胞标记doublecortin（DC）和Distalless-related homeobox（DLX）或少突胶质细胞祖细胞标记Nkx2.2。在吻侧迁移流（RMS）中，EGFP（+）细胞显示迁移表型。这些细胞的一部分是NG 2（-）/Er 81（+）/DC+/DLX+或NG 2（+）/Nkx2.2（+）。将DiI（1，1 '-双十八烷基-3，3，3'，3 '-四甲基吲哚羰花青高氯酸盐）注射到出生后早期小鼠的侧脑室（LV）中，证实NG 2（+）/EGFP（+）祖细胞从SVZ通过RMS迁移到OB。此外，移植到出生后早期LV中的荧光激活细胞分选纯化的NG 2（+）/CNP-EGFP（+）或NG 2（+）/β-肌动蛋白增强的黄色荧光蛋白阳性（EYFP+）祖细胞显示出广泛的吻侧和尾侧迁移。RMS内EYFP+或EGFP（+）移植物来源的细胞为DLX+/Er 81（+）或Nkx2.2（+），迁移至OB，并分化为中间神经元和少突胶质细胞。在皮质下白色物质（SCWM）中，移植细胞分化为少突胶质细胞或星形胶质细胞。移植从SVZ选择性纯化的NG 2（+）/EYFP+祖细胞显示这些细胞是迁移的，并在OB、海马和纹状体中产生胶质细胞和神经元。相反，皮质、OB或小脑NG 2（+）细胞的迁移能力非常有限，并在SCWM和纹状体中产生胶质细胞。我们的研究结果表明NG 2（+）祖细胞之间存在区域特异性差异，并表明NG 2（+）细胞可以在整个RMS中迁移，并有助于出生后OB中的胶质细胞和神经发生。
We used a 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNP)-enhanced green fluorescent protein (EGFP) transgenic mouse to study postnatal subventricular zone (SVZ) progenitor fate, with a focus on the olfactory bulb (OB). The postnatal OB of the CNP-EGFP mouse contained EGFP(+) interneurons and oligodendrocytes. In the anterior SVZ, the majority of EGFP(+) progenitors were NG2(+). These NG2(+)/EGFP(+) progenitors expressed the OB interneuron marker Er81, the neuroblast markers doublecortin (DC) and Distalless-related homeobox (DLX), or the oligodendrocyte progenitor marker Nkx2.2. In the rostral migratory stream (RMS), EGFP(+) cells displayed a migrating phenotype. A fraction of these cells were either NG2(-)/Er81(+)/DC+/DLX+ or NG2(+)/Nkx2.2(+). DiI (1,1'-dioctadecyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorate) injection into the lateral ventricle (LV) of early postnatal mice demonstrated that NG2 (+)/EGFP(+) progenitors migrate from the SVZ through the RMS into the OB. Moreover, fluorescence-activated cell-sorting- purified NG2(+)/CNP-EGFP (+) or NG2(+)/beta-actin-enhanced yellow fluorescent protein-positive (EYFP+) progenitors transplanted into the early postnatal LV displayed extensive rostral and caudal migration. EYFP+ or EGFP (+) graft-derived cells within the RMS were DLX+/ Er81(+) or Nkx2.2(+), migrated to the OB, and differentiated to interneurons and oligodendrocytes. In the subcortical white matter (SCWM), grafted cells differentiated to either oligodendrocytes or astrocytes. Transplantation of NG2(+)/EYFP+ progenitors selectively purified from the SVZ showed that these cells were migratory and generated glia and neurons in the OB, hippocampus, and striatum. In contrast, cortical, OB, or cerebellar NG2(+) cells had a very limited migratory potential and gave rise to glia in the SCWM and striatum. Our findings indicate region-specific differences between NG2(+) progenitor cells and show that NG2(+) cells can migrate throughout the RMS and contribute to both gliogenesis and neurogenesis in the postnatal OB.