Phylogeographic analysis of mitochondrial DNA in northern Asian Populations
Phylogeographic analysis of mitochondrial DNA in northern Asian Populations
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DOI：
10.1086/522933
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发表时间：
2007-11-01
影响因子：
9.8
通讯作者：
Zakharov, Ilia
中科院分区：
文献类型：
--
作者：
Derenko, Miroslava;Malyarchuk, Boris;Zakharov, Ilia
为了阐明人类在北方亚洲的殖民过程和人类向美洲的扩散，从北方、中部、东部和西南部亚洲的18个土著人群中采集的1,432个控制区序列中选择了71个线粒体DNA（mtDNA）谱系的不同子集进行全基因组测序。在线粒体DNA全序列测定的基础上，我们修订了单倍群A、D2、G1、M7和I的分类;鉴定了6个新的单倍群亚群（I4、N1 e、G1 c、M7 d、M7 e和J1 b2 a）;并对单倍群N1 a和G1 b进行了充分的鉴定，这两个单倍群以前仅通过第一高变片段（HVS 1）测序和编码区限制性片段长度多态性分析进行了描述。我们的研究结果表明，西伯利亚南部的mtDNA池窝藏与旧石器时代晚期和/或新石器时代早期分散从东亚和亚洲西南部/南高加索地区的几个谱系。此外，D2谱系的地理分布表明，西伯利亚南部很可能是该亚单倍群在冰后期最大规模向北方西伯利亚扩散的地理来源，D2 b分支的扩张发生在白令海，与7,000年前相似。总的来说，对北方亚洲人mtDNA基因库的详细分析提供了额外的证据，排除了人类最初在亚洲殖民的北方亚洲路线的存在。
To elucidate the human colonization process of northern Asia and human dispersals to the Americas, a diverse subset of 71 mitochondrial DNA (mtDNA) lineages was chosen for complete genome sequencing from the collection of 1,432 control-region sequences sampled from 18 autochthonous populations of northern, central, eastern, and southwestern Asia. On the basis of complete mtDNA sequencing, we have revised the classification of haplogroups A, D2, G1, M7, and I; identified six new subhaplogroups (I4, N1e, G1c, M7d, M7e, and J1b2a); and fully characterized haplogroups N1a and G1b, which were previously described only by the first hypervariable segment (HVS1) sequencing and coding-region restriction-fragment-length polymorphism analysis. Our findings indicate that the southern Siberian mtDNA pool harbors several lineages associated with the Late Upper Paleolithic and/or early Neolithic dispersals from both eastern Asia and southwestern Asia/southern Caucasus. Moreover, the phylogeography of the D2 lineages suggests that southern Siberia is likely to be a geographical source for the last postglacial maximum spread of this subhaplogroup to northern Siberia and that the expansion of the D2b branch occurred in Beringia similar to 7,000 years ago. In general, a detailed analysis of mtDNA gene pools of northern Asians provides the additional evidence to rule out the existence of a northern Asian route for the initial human colonization of Asia.