Evidence for distinct populations of human Merkel cells
Evidence for distinct populations of human Merkel cells
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DOI：
10.1007/s00418-009-0578-0
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发表时间：
2009-07-01
影响因子：
2.3
通讯作者：
Moll, Ingrid
中科院分区：
文献类型：
--
作者：
Eispert, A. -C.;Fuchs, F.;Moll, Ingrid
默克尔细胞（MC）是位于皮肤中来源不明的神经内分泌细胞。它们在电子显微镜水平上通过电子致密颗粒进行鉴定，在光学显微镜水平上通过细胞角蛋白8、18、19和20的存在来鉴定。关于上皮和神经起源的其他分子的存在的相互矛盾的报道促使我们研究是否存在不同的人类MCs群体。在这里，我们展示了绒毛蛋白、N-CAM、NGF-R 和神经丝在 MC 中的异质表达。突触素存在于所有 MC 中，但强度不同，巢蛋白不存在。毛囊间表皮、毛囊和无毛表皮之间的表达模式有所不同。我们得出的结论是，存在不同的 MC 群体，但所有群体都含有上皮细胞和神经细胞的标记。讨论了不同群体的假定功能。
Merkel cells (MCs) are neuroendocrine cells of unknown origin located in the skin. They are identified at electron microscopic level by electron dense granules, at light microscopic level by the presence of cytokeratins 8, 18, 19 and 20. Contradictory reports concerning the presence of other molecules of epithelial as well as neural origin prompted us to investigate whether there are distinct populations of human MCs. Here, we show the heterogeneous expression of villin, N-CAM, NGF-R, and neurofilaments in MCs. Synaptophysin is found in all MCs but with different intensity, nestin is absent. Expression patterns vary between interfollicular epidermis, hair follicles and glabrous epidermis. We conclude that there are distinct populations of MCs, but all populations contain markers for epithelial as well as neural cells. Putative functions of the distinct populations are discussed.