Effect of Polyelectrolyte Multilayers Assembled on Ordered Nanostructures on Adhesion of Human Fibroblasts.
Effect of Polyelectrolyte Multilayers Assembled on Ordered Nanostructures on Adhesion of Human Fibroblasts.
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DOI：
10.1021/acsami.6b09349
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发表时间：
2016-09
影响因子：
9.5
通讯作者：
Marcus S Niepel;J. Mano;T. Groth
中科院分区：
文献类型：
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作者：
Marcus S Niepel;J. Mano;T. Groth
本文首次将纳米球光刻技术（NSL）和逐层技术（LbL）相结合，设计了一种灵活的系统来实现细胞粘附的纳米形貌控制。NSL用于生成不同尺寸和距离的四面体金纳米点的规则图案。除了改变形貌外，LbL还用于在金点顶部生成由肝素（HEP）和聚乙烯亚胺（PEI）组成的聚电解质多层（PEM）体系。通过预先钝化周围的硅或玻璃表面，在金点上局部形成PEM。通过将HEP溶液的pH值调整为酸性或碱性值可以改变PEM的性质。对人真皮成纤维细胞（HDF）的研究表明，细胞在pH 5.0形成的PEM上的扩散程度更高，这与结构尺寸有关。此外，纳米结构上的细胞丝状足形成高度增加，显示HEP作为终端层。由于其高度的化学和地形异质性，新系统为指导干细胞分化提供了巨大的潜力。
Nanosphere lithography (NSL) and the layer-by-layer (LbL) technique are combined here for the first time to design a flexible system to achieve nanotopographical control of cell adhesion. NSL is used to generate regular patterns of tetrahedral gold nanodots of different size and distance. Besides the change in topography, LbL is used to generate a polyelectrolyte multilayer (PEM) system consisting of heparin (HEP) and poly(ethylene imine) (PEI) on top of the gold dots. The localized formation of PEM on gold dots is achieved by prior passivation of the surrounding silicon or glass surface. Properties of PEM are changed by adjusting the pH value of HEP solution to either acidic or alkaline values. Studies with human dermal fibroblasts (HDF) reveal that cells spread to a higher extent on PEM formed at pH 5.0 in dependence on the structure dimension. Further, filopodia formation is highly increased in cells on nanostructures exhibiting HEP as a terminal layer. The new system offers a great potential to guide stem cell differentiation in the future owing to its high degree of chemical and topographical heterogeneity.