Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1.
Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1.
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DOI：
10.4049/jimmunol.130.5.2133
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发表时间：
1983-05
影响因子：
4.4
通讯作者：
B. Perussia;S. Starr;S. Abraham;V. Fanning;G. Trinchieri
中科院分区：
文献类型：
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作者：
B. Perussia;S. Starr;S. Abraham;V. Fanning;G. Trinchieri
我们描述了单克隆抗体B73.1的生产，与人类淋巴细胞的一个子集，并在约一半的捐助者，与嗜中性多形核白细胞。在正常成人供体的外周血中，14.6 +/- 8.5%的淋巴细胞与B73.1抗体反应。B73.1（+）淋巴细胞亚群不携带典型T或B细胞的标志物，对应于含有抗体依赖性杀伤（K）和自然杀伤（NK）细胞的淋巴细胞亚群。我们证明：a）几乎所有具有K/NK细胞毒性活性的淋巴细胞都存在于携带B73.1确定的抗原的淋巴细胞亚群中; B）B73.1（+）淋巴细胞亚群携带已知存在于K/NK细胞上的抗原的组合;和c）细胞毒性水平与个体供体中B73.1（+）淋巴细胞的实际数量之间存在正相关性。我们还报告了根据供体年龄和组织类型的B73.1（+）淋巴细胞的分布。使用的B73.1抗体在定量的K/NK细胞的实际数量，并在执行自发细胞毒性的功能研究进行了讨论。
We describe the production of the monoclonal antibody B73.1, reacting with a subset of human lymphocytes and, in about one-half of the donors, with neutrophilic polymorphonuclear leukocytes. In the peripheral blood from normal adult donors, 14.6 +/- 8.5% of the lymphocytes react with B73.1 antibody. The B73.1(+) lymphocyte subset does not bear markers of typical T or B cells and corresponds to the lymphocyte subset containing antibody-dependent killer (K) and natural killer (NK) cells. We demonstrate that: a) virtually all lymphocytes with K/NK cytotoxic activity are found in the lymphocyte subpopulation bearing the B73.1-defined antigen; b) the B73.1(+) lymphocyte subset bears the combination of antigens known to be present on K/NK cells; and c) there is a positive correlation between the level of cytotoxicity and the actual number of B73.1(+) lymphocytes in individual donors. We also report the distribution of B73.1(+) lymphocytes according to donor age and tissue types. The use of the B73.1 antibody in quantitating the actual number of K/NK cells and in performing functional studies on spontaneous cytotoxicity is discussed.