DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair
DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair
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DOI：
10.1101/cshperspect.a016444
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发表时间：
2015-02-01
影响因子：
7.2
通讯作者：
Morrical, Scott W.
中科院分区：
文献类型：
--
作者：
Morrical, Scott W.
异双工DNA的形成是通过同源重组交换DNA序列的核心步骤，也是通过同源定向修复途径精确修复断裂染色体的核心步骤。在细胞中，异双工DNA主要是通过促进DNA配对和退火反应的重组蛋白活动产生的。参与配对和退火的蛋白质包括reca家族DNA配对蛋白、单链DNA （ssDNA）结合蛋白、重组中介蛋白、退火蛋白和核酸酶。这篇综述探讨了这些配对和退火蛋白的特性，并强调了它们在复杂重组过程中的作用，包括双霍利迪结（DhJ）形成、合成依赖的链退火和单链退火途径- dna交易，这对个体生物的基因组稳定性和物种进化都至关重要。
The formation of heteroduplex DNA is a central step in the exchange of DNA sequences via homologous recombination, and in the accurate repair of broken chromosomes via homology-directed repair pathways. In cells, heteroduplex DNA largely arises through the activities of recombination proteins that promote DNA-pairing and annealing reactions. Classes of proteins involved in pairing and annealing include RecA-family DNA-pairing proteins, single-stranded DNA (ssDNA)-binding proteins, recombination mediator proteins, annealing proteins, and nucleases. This review explores the properties of these pairing and annealing proteins, and highlights their roles in complex recombination processes including the double Holliday junction (DhJ) formation, synthesis-dependent strand annealing, and single-strand annealing pathways-DNA transactions that are critical both for genome stability in individual organisms and for the evolution of species.