Roles of rpoN, fliA, and flgR in expression of flagella in Campylobacter jejuni
Roles of rpoN, fliA, and flgR in expression of flagella in Campylobacter jejuni
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DOI：
10.1128/jb.183.9.2937-2942.2001
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发表时间：
2001-05-01
影响因子：
3.2
通讯作者：
Penn, CW
中科院分区：
文献类型：
--
作者：
Jagannathan, A;Constantinidou, C;Penn, CW
研究了空肠弯曲菌NCTC 11168基因组序列中存在的三个潜在的鞭毛表达调控因子rpoN、flgR和fliA，它们分别编码另类sigma因子sigma(54)、与sigma(54)相关的转录激活因子flgR和鞭毛sigma因子sigma(28)。这三个基因在空肠弯曲菌NCTC 11168和NCTC 11828中插入失活。对野生型和突变型菌株的电子显微镜研究表明，rpoN和flgR突变体是非鞭毛的，而fliA突变体截短了鞭毛。三个突变体的免疫印迹实验证实了rpoN、flgR和fliA在鞭毛蛋白表达中的作用。
Three potential regulators of flagellar expression present in the genome sequence of Campylobacter jejuni NCTC 11168, the genes rpoN,flgR, and fliA, which encode the alternative sigma factor sigma (54), the sigma (54)-associated transcriptional activator FlgR, and the flagellar sigma factor sigma (28), respectively, were investigated for their role in global regulation of flagellar expression. The three genes were insertionally inactivated in C. jejuni strains NCTC 11168 and NCTC 11828. Electron microscopic studies of the wild-type and mutant strains showed that the rpoN and flgR mutants were nonflagellate and that the fliA mutant had truncated flagella. Immunoblotting experiments with the three mutants confirmed the roles of rpoN, flgR, and fliA in the expression of flagellin.