Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense.
Insights into the ancestry evolution of the Mycobacterium tuberculosis complex from analysis of Mycobacterium riyadhense.
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从利雅得分枝杆菌的分析了解结核分枝杆菌复合体的祖先进化。
DOI：
10.1093/nargab/lqab070
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发表时间：
2021-09
影响因子：
4.6
通讯作者：
Pain A
中科院分区：
文献类型：
--
作者：
Guan Q;Garbati M;Mfarrej S;AlMutairi T;Laval T;Singh A;Fagbo S;Smyth A;Browne JA;urRahman MA;Alruwaili A;Hoosen A;Meehan CJ;Nakajima C;Suzuki Y;Demangel C;Bhatt A;Gordon SV;AlAsmari F;Pain A
目前的进化情景表明，结核分枝杆菌是通过基因组适应的累积过程从环境中进化出来的。利雅得分枝杆菌是一种相关的杆菌，在世界各地越来越多地从具有结核样症状的人类临床病例中分离出来。为了阐明M. riyadhense和其他分枝杆菌物种，包括M.结核复合群（MTBC），8株临床分离的M. riyadhense进行测序和分析。我们表明，除其他功能外，M。riyadhense与M.与其他非结核分枝杆菌相比，显示毒素/抗毒素对、PE/PPE家族蛋白的扩增。我们观察M。riyadhense缺乏wecE基因，这可能导致缺乏脂寡糖（LOS）IV。感染的巨噬细胞的比较转录组学分析显示，编码I型IFN应答诱导物的基因，如胞质DNA传感器，在感染M. riyadhense或M. kansasii与BCG或M.结核总的来说，我们的工作为M. riyadhense，它与MTBC的关系，以及它作为研究分枝杆菌毒力和致病机制的系统的潜力。
Current evolutionary scenarios posit the emergence of Mycobacterium tuberculosis from an environmental saprophyte through a cumulative process of genome adaptation. Mycobacterium riyadhense, a related bacillus, is being increasingly isolated from human clinical cases with tuberculosis-like symptoms in various parts of the world. To elucidate the evolutionary relationship between M. riyadhense and other mycobacterial species, including members of the M. tuberculosis complex (MTBC), eight clinical isolates of M. riyadhense were sequenced and analyzed. We show, among other features, that M. riyadhense shares a large number of conserved orthologs with M. tuberculosis and shows the expansion of toxin/antitoxin pairs, PE/PPE family proteins compared with other non-tuberculous mycobacteria. We observed M. riyadhense lacks wecE gene which may result in the absence of lipooligosaccharides (LOS) IV. Comparative transcriptomic analysis of infected macrophages reveals genes encoding inducers of Type I IFN responses, such as cytosolic DNA sensors, were relatively less expressed by macrophages infected with M. riyadhense or M. kansasii, compared to BCG or M. tuberculosis. Overall, our work sheds new light on the evolution of M. riyadhense, its relationship to the MTBC, and its potential as a system for the study of mycobacterial virulence and pathogenesis.