Diosgenin inhibits melanogenesis through the activation of phosphatidylinositol-3-kinase pathway (PI3K) signaling
Diosgenin inhibits melanogenesis through the activation of phosphatidylinositol-3-kinase pathway (PI3K) signaling
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DOI：
10.1016/j.lfs.2007.05.009
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发表时间：
2007-06-27
期刊：
影响因子：
6.1
通讯作者：
Park, Deokhoon
中科院分区：
文献类型：
--
作者：
Lee, Jongsung;Jung, Kwangseon;Park, Deokhoon
黑色素水平升高是许多皮肤病的特征，包括获得性色素沉着，如黄褐斑、炎症后黑皮病和太阳斑。因此，开发脱色剂的需求越来越大。为了评价薯蓣皂苷元的脱色能力并阐明其作用机制，我们在B16黑色素瘤细胞中进行了实验。本研究评估了黑色素含量和参与黑色素形成的蛋白质的Western blots。薯蓣皂苷元对黑色素含量有显著抑制作用。为了阐明薯蓣皂苷元的脱色机制，我们研究了薯蓣皂苷元在磷脂酰肌醇-3激酶（PI3K）通路中的作用。在本研究中，薯蓣皂苷元抑制PI3K抑制剂LY294002诱导的Akt和GSK 3 β磷酸化的降低。根据这一结果，薯蓣皂苷元增加了MITF（小眼相关转录因子）和酪氨酸酶的产生水平。这些数据表明薯蓣皂苷元通过激活PI3K通路抑制黑色素生成。在B16黑色素瘤细胞中，LY294002增加的黑色素生成水平被薯蓣皂苷元降低，这一事实进一步证实了这一观点。我们的研究表明薯蓣皂苷元通过激活PI3K通路抑制黑色素生成，也提示薯蓣皂苷元可能是色素沉着的有效抑制剂。(C) 2007爱思唯尔公司版权所有。
An increased level of melanin is characteristic of a large number of skin diseases, including acquired hyperpigmentation conditions such as melasma, postinflammatory melanoderma, and solar lentigo. Thus, there is an increasing need for the development of depigmenting agents. In order to evaluate the depigmenting capacity of diosgenin and elucidate its mechanism of action, several experiments were performed in B16 melanoma cells. Melanin content and Western blots for proteins that are involved in melanogenesis were assessed in this study. The melanin content was significantly inhibited by diosgenin. To clarify the mechanism of the depigmenting property of diosgenin, we examined the involvement of diosgenin in the phosphatidylinositol-3-kinase (PI3K) pathway. In this study, diosgenin inhibited the reduction of Akt and GSK 3 beta phosphorylation induced by LY294002, a PI3K inhibitor. In accordance with this result, production levels of MITF (microphthalmia-associated transcription factor) and tyrosinase were increased by diosgenin. These data suggest that diosgenin inhibits melanogenesis through the activation of the PI3K pathway. This suggestion was further confirmed by the fact that the increased production level of melanin by LY294002 was reduced by diosgenin in B16 melanoma cells. Our study shows that diosgenin inhibits melanogenesis by activating the PI3K pathway, and also suggests that diosgenin may be an effective inhibitor of hyperpigmentation. (C) 2007 Elsevier Inc. All rights reserved.