High Mobility Group Box 1 (HMGB1) Protein is Present in the Cerebrospinal Fluid of Dogs with Encephalitis
High Mobility Group Box 1 (HMGB1) Protein is Present in the Cerebrospinal Fluid of Dogs with Encephalitis
复制标题
DOI：
10.1292/jvms.10-0444
复制
发表时间：
2011-07-01
影响因子：
1.2
通讯作者：
Yokota, Hiroshi
中科院分区：
文献类型：
--
作者：
Miyasho, Taku;Nakamura, Kozo;Yokota, Hiroshi
在特定品种的小型犬中报告了许多病因不明的脑炎病例。高迁移率族蛋白1（HMGB1）是近年来发现的一种新的神经元坏死和炎症的标志物。本研究的目的是确定是否HMGB1水平升高的脑脊液（CSF）的狗怀疑有脑炎。从31只通过临床检查和磁共振成像（MRI）扫描诊断为脑炎疾病的犬中获得CSF。CSF样本通过蛋白质印迹法（WB）和酶联免疫吸附试验（ELISA）与HMGB1的多克隆抗体进行分析。脑炎犬血清中HMGB1浓度显著高于健康对照犬。HMGB1的浓度与细胞计数和总蛋白浓度相关，这是与脑炎相关的神经元炎症的已知CSF指标。这些结果表明，CSF中的HMGB1蛋白证实了大多数犬脑炎病例中存在坏死和炎症，HMGB1将成为脑炎的新指标。
Many cases of encephalitic disease with unknown etiologies have been reported in specific breeds of small dogs. High mobility group box 1 (HMGB1) in neuronal cells was recently found to be a novel cytokine-like mediator that is a marker of neuronal necrosis and inflammation. The aim of this study was to determine whether HMGB1 levels are elevated in the cerebrospinal fluid (CSF) of dogs suspected of having encephalitis. CSF was obtained from 31 dogs that were diagnosed with an encephalitic disease by clinical examinations and magnetic resonance image (MRI) scanning. The CSF samples were analyzed via western blotting (WB) and an enzyme-linked immunosorbent assay (ELISA) with a polyclonal antibody against HMGB1. The mean HMGB1 concentration was significantly higher in the encephalitic dogs than that in the healthy controls. The concentrations of HMGB1 were correlated with the cell counts and total protein concentrations, which are known CSF indicators of the neuronal inflammation associated with encephalitis. These results suggest that HMGB1 protein in CSF confirms the presence of necrosis and inflammation in most cases of canine encephalitis and that HMGB1 will be a new indicator of encephalitis.