Soft matter perspective on protein crystal assembly
Soft matter perspective on protein crystal assembly
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DOI：
10.1016/j.colsurfb.2015.07.023
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发表时间：
2016-01-01
影响因子：
5.8
通讯作者：
Charbonneau, Patrick
中科院分区：
文献类型：
--
作者：
Fusco, Diana;Charbonneau, Patrick
晶体学可能是蛋白质结构测定的黄金标准，但获得必要的高质量晶体在某些方面也类似于勘探贵金属。在软物质物理学中开发的用于理解胶体组装的工具和模型为蛋白质结晶问题提供了一些见解。这篇专题评论描述了在这种情况下蛋白质和胶体之间做出的各种类比。我们强调了补丁颗粒模型的解释能力，但也面临着为结晶特定蛋白质提供指导的挑战。最后，我们提出了未来可能的研究方向。这篇评论的目的是为软物质科学家感兴趣的蛋白质结晶作为一个自组装问题，并作为一个介绍相关的物理文献的蛋白质科学家更普遍。(C)2015 Elsevier B.V.版权所有。
Crystallography may be the gold standard of protein structure determination, but obtaining the necessary high-quality crystals is also in some ways akin to prospecting for the precious metal. The tools and models developed in soft matter physics to understand colloidal assembly offer some insights into the problem of crystallizing proteins. This topical review describes the various analogies that have been made between proteins and colloids in that context. We highlight the explanatory power of patchy particle models, but also the challenges of providing guidance for crystallizing specific proteins. We conclude with a presentation of possible future research directions. This review is intended for soft matter scientists interested in protein crystallization as a self-assembly problem, and as an introduction to the pertinent physics literature for protein scientists more generally. (C) 2015 Elsevier B.V. All rights reserved.