Polycomb safeguards imaginal disc specification through control of the Vestigial-Scalloped complex.
Polycomb safeguards imaginal disc specification through control of the Vestigial-Scalloped complex.
复制标题
Polycomb 通过控制残留-扇形复合体来保护成虫盘的规格。
DOI：
10.1101/2023.04.11.536444
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Kumar,JustinP
中科院分区：
文献类型：
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作者：
Brown,HaleyE;Weasner,BrandonP;Weasner,BonnieM;Kumar,JustinP
发育生物学的一个基本目标是了解细胞和组织的命运是如何被指定的。果蝇的想象椎间盘是解决这一范式的优秀模型系统，因为当椎间盘在损伤后再生或当关键选择基因被错误调控时，它们的命运可以被重定向。在这里，我们表明当polycomexpression减少时，翅膀选择器在遗传上被异位激活。这导致了退化-扇贝复合体的不适当形成，迫使眼睛转变成翅膀。我们进一步证明，破坏这个复合体并不仅仅是阻止翅膀的形成或恢复眼睛的发育。相反，免疫组织化学和高通量基因组分析表明，眼触角盘出乎意料地经历了增生生长，多个结构域被组织到其他图像盘和组织中。这些发现提供了对复杂的发育景观的见解，组织在采用最终命运之前必须导航。
A fundamental goal of developmental biology is to understand how cell and tissue fates are specified. The imaginal discs ofDrosophilaare excellent model systems for addressing this paradigm as their fate can be redirected when discs regenerate after injury or when key selector genes are misregulated. Here, we show that whenPolycombexpression is reduced, the wing selector genevestigialis ectopically activated. This leads to the inappropriate formation of the Vestigial–Scalloped complex, which forces the eye to transform into a wing. We further demonstrate that disrupting this complex does not simply block wing formation or restore eye development. Instead, immunohistochemistry and high-throughput genomic analysis show that the eye-antennal disc unexpectedly undergoes hyperplastic growth with multiple domains being organized into other imaginal discs and tissues. These findings provide insight into the complex developmental landscape that tissues must navigate before adopting their final fate.