Origin of the cytoplasmic pH changes during anaerobic stress in higher plant cells. Carbon-13 and phosphorous-31 nuclear magnetic resonance studies
Origin of the cytoplasmic pH changes during anaerobic stress in higher plant cells. Carbon-13 and phosphorous-31 nuclear magnetic resonance studies
复制标题
DOI：
10.1104/pp.125.2.912
复制
发表时间：
2001-02-01
期刊：
影响因子：
7.4
通讯作者：
Bligny, R
中科院分区：
文献类型：
--
作者：
Gout, E;Boisson, AM;Bligny, R
我们测试了缺氧西卡莫尔（Acer pseudoplatanus）植物细胞酸中毒中三磷酸核苷（NTP）水解、乙醇和有机酸合成以及H+泵ATP酶活性的贡献。选择培养细胞以改变NTP池和用特定营养培养基（磷酸盐[Pi]-剥夺和腺嘌呤-或甘油-供应）发酵。在体内P-31-和C-13-核磁共振（NMR）光谱被用来非侵入性地测量细胞内的pH值，Pi，磷酸单酯，核苷酸，乳酸，和乙醇。缺氧后，细胞质（cyt）pH值（7.5）下降到6.8 4至5分钟内，而液泡pH值（5.7）和外部pH值（6.5）保持稳定。NTP池同时从210降至
We tested the contribution of nucleoside triphosphate (NTP) hydrolysis, ethanol, and organic acid syntheses, and H+-pump ATPases activity in the acidosis of anoxic sycamore (Acer pseudoplatanus) plant cells. Culture cells were chosen to alter NTP pools and fermentation with specific nutrient media (phosphate [Pi]-deprived and adenine- or glycerol-supplied). In vivo P-31- and C-13-nuclear magnetic resonance (NMR) spectroscopy was utilized to noninvasively measure intracellular pHs, Pi, phosphomonoesters, nucleotides, lactate, and ethanol. Following the onset of anoxia, cytoplasmic (cyt) pH (7.5) decreased to 6.8 within 4 to 5 min, whereas vacuolar pH (5.7) and external pH (6.5) remained stable. The NTP pool simultaneously decreased from 210 to