Urothelial Differences in the Exstrophy-Epispadias Complex: Potential Implications for Management
Urothelial Differences in the Exstrophy-Epispadias Complex: Potential Implications for Management
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DOI：
10.1097/ju.0000000000001510
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发表时间：
2021-05-01
影响因子：
6.6
通讯作者：
Gearhart, John P.
中科院分区：
文献类型：
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作者：
Kasprenski, Matthew;Michaud, Jason;Gearhart, John P.
目的：作者研究了尿道外裂-尿道上裂复合症患者的尿道组织学差异和尿路上皮分化的终末标志物的表达。材料和方法：2012年至2017年，从69例小儿尿道外裂-尿道上裂复合症患者中获得膀胱活检。将这些标本与正常对照的膀胱标本进行比较。所有膀胱标本均进行组织学评估，然后进行尿斑蛋白II和p63免疫组化染色。表达水平uroplakin-II和p63，然后由一个盲目的pathologist.Results：四十三个经典的膀胱外翻活检（10个新生儿关闭，22延迟关闭，和11个重复关闭）进行了评估。另外还对18例泄殖腔外翻患者和8例尿道上裂患者的活检进行了评价。将这些标本与8个正常对照膀胱标本进行比较。总的来说，与对照组相比，外痔-尿道上裂复合症患者的尿斑蛋白-II表达较低（p
Purpose: The authors examined the urothelium of exstrophy-epispadias complex spectrum patients for histological differences and expression of terminal markers of urothelial differentiation.Materials and Methods: Between 2012 and 2017 bladder biopsies were obtained from 69 pediatric exstrophy-epispadias complex patients. These specimens were compared to bladder specimens from normal controls. All bladder specimens underwent histological assessment followed by immunohistochemical staining for uroplakin-II and p63. Expression levels of uroplakin-II and p63 were then assessed by a blinded pathologist.Results: Forty-three classic bladder exstrophy biopsies were obtained (10 newborn closures, 22 delayed closures, and 11 repeat closures). Additional biopsies from 18 cloacal exstrophy patients and 8 epispadias patients were also evaluated. These specimens were compared to 8 normal control bladder specimens. Overall, uroplakin-II expression was lower in exstrophy-epispadias complex patients compared to controls (p