MinION as part of a biomedical rapidly deployable laboratory
MinION as part of a biomedical rapidly deployable laboratory
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DOI：
10.1016/j.jbiotec.2016.12.006
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发表时间：
2017-05-20
影响因子：
4.1
通讯作者：
Antwerpen, Markus H.
中科院分区：
文献类型：
--
作者：
Walter, Mathias C.;Zwirglmaier, Katrin;Antwerpen, Markus H.
快速的周转时间对于生物医学侦察至关重要，特别是对于危险的病原体。测序技术及其设备的最新进展允许在靠近疫情中心的固定实验室外短时间内进行测序。在我们的研究中，我们评估了便携式测序设备 MinION，作为专门用于识别高致病性病原体的快速部署实验室的一部分。我们在炎热气候条件下的可部署现场实验室中进行北约真人演习时测试了该设备。这些样本是从生物恐怖场景中获得的，该场景是演习的一部分，其中含有未知的细菌制剂。为了模拟资源有限的远程部署站点的条件，我们在没有互联网访问的情况下操作定序器。使用宏基因组方法，我们能够识别分析样本中的病原体。此外，根据获得的数据，我们能够进行低至菌株水平的分子分型。在我们的研究中，我们挑战了该设备并讨论了在固定实验室之外对生物样本进行测序的进展以及仍然存在的局限性。然而，大规模并行测序作为一种非选择性方法可以产生重要信息，并且能够支持疫情调查——即使是在现场。皇冠版权所有 (C) 2016 由 Elsevier B.V. 出版。保留所有权利。
Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak.In our study, we evaluated the portable sequencing device MinION as part of a rapidly deployable laboratory specialized in identification of highly pathogenic agents. We tested the device in the course of a NATO live agent exercise in a deployable field laboratory in hot climate conditions. The samples were obtained from bio-terroristic scenarios that formed part of the exercise and contained unknown bacterial agents. To simulate conditions of a resource-limited remote deployment site, we operated the sequencer without internet access. Using a metagenomic approach, we were able to identify the causative agent in the analyzed samples. Furthermore, depending on the obtained data, we were able to perform molecular typing down to strain level.In our study we challenged the device and discuss advances as well as remaining limitations for sequencing biological samples outside of stationary laboratories. Nevertheless, massive parallel sequencing as a non-selective methodology yields important information and is able to support outbreak investigation - even in the field. Crown Copyright (C) 2016 Published by Elsevier B.V. All rights reserved.