HISTOTYPIC DIFFERENTIATION OF NEONATAL MOUSE RETINA IN ORGAN-CULTURE
HISTOTYPIC DIFFERENTIATION OF NEONATAL MOUSE RETINA IN ORGAN-CULTURE
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DOI：
10.3109/02713688908997401
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发表时间：
1989-10-01
影响因子：
2
通讯作者：
SANYAL, S
中科院分区：
文献类型：
--
作者：
CAFFE, AR;VISSER, H;SANYAL, S
将新生小鼠的视网膜全部移植，无论有或没有视网膜色素上皮 (RPE) 和相邻的间充质细胞，并在器官培养物中维持长达 3 周。外植体保持平坦，莲座丛形成极少，组织发生变化按正常顺序进行。体外11、14和21天后，三个细胞层——包括分化良好的视杆细胞和视锥细胞周体的外核层、内核层和带有丛状层的神经节细胞层——与体内眼睛的细胞层相当。电子显微镜分析显示，在没有 RPE 的外植体中，核层像体内一样发育，但没有形成受体外节 (ROS)。当 RPE 存在时，受体内节段表现正常，并且出现包括大量椎间盘结构的 ROS。还认识到突触元件的存在。间充质细胞在存在时分化为脉络膜和巩膜组织，并且似乎对 RPE 细胞发挥支持作用。详细描述了该系统，并讨论了其对分析视网膜发育中各种细胞和代谢因素的适用性。
Retinae from neonatal mice were explanted in toto, with or without the retinal pigment epithelium (RPE) and adjoining mesenchymal cells, and maintained in organ culture for up to 3 weeks. The explants remained flat, rosette formation was minimal and histogenetic changes followed in the normal sequence. After 11, 14 and 21 days in vitro the three cellular layers the outer nuclear layer including well differentiated rod and cone perikarya, the inner nuclear layer and the ganglion cell layer with the intervening plexiform layers were comparable to those of the in vivo eyes. Electron microscopic analysis revealed that in the explants without RPE the nuclear layers devleoped as in vivo, but receptor outer segments (ROS) were not formed. When the RPE was present, receptor inner segments appeared normal and ROS including profuse disc structures were developed. Presence of synaptic elements was also recognized. Mesenchymal cells, when present differentiated into choroidal and scleral tissues and appeared to play a supportive role for the RPE cells. The system is described in detail and its suitability for the analysis of various cellular and metabolic factors in the development of the retina is discussed.