Microarray analysis of IL-1 β-stimulated chemokine genes in synovial fibroblasts from human TMJ
Microarray analysis of IL-1 β-stimulated chemokine genes in synovial fibroblasts from human TMJ
复制标题
DOI：
10.1111/j.1600-0714.2007.00515.x
复制
发表时间：
2007-04-01
影响因子：
3.3
通讯作者：
Kondoh, Toshirou
中科院分区：
文献类型：
--
作者：
Ogura, Naomi;Akutsu, Miwa;Kondoh, Toshirou
背景：白细胞介素 (IL)-1 beta 被认为在颞下颌关节 (TMJ) 的多种病理状况中发挥着关键作用。通过寡核苷酸微阵列分析研究了IL-1β刺激的滑膜成纤维细胞的基因表达谱，以阐明与TMJ囊内病理状况相关的候选基因。方法：从IL-1β治疗后的5名患者的滑膜成纤维细胞中分离RNA。使用 GeneChip 进行基因表达谱分析。通过比较 IL-1 β 处理的细胞与未处理的细胞来确定基因表达的变化。 结果：在五个实验中，未处理的滑膜成纤维细胞和 IL-1 β 处理的滑膜成纤维细胞之间总共 121 个基因的平均强度存在三倍以上的差异。上调最多的 10 个基因中有 5 个趋化因子，其中上调最多的基因是 CCL20。 121 个 IL-1 β 反应基因包括 12 个趋化因子，其 mRNA 水平通过实时 PCR 得到证实。结论：这些数据应该提供有关 TMJ 病理状况的有用信息，特别是支持 TMJ 的诊断和治疗方法。
BACKGROUND: Interleukin (IL)-1 beta is thought to play a key role in several pathologic conditions of the temporomandibular joint (TMJ). Gene expression profile of synovial fibroblasts stimulated with IL-1 beta was studied by oligonucleotide microarray analysis to elucidate candidate genes associated with intracapsular pathologic conditions of TMJ.METHODS: RNA was isolated from synovial fibroblasts from five patients after IL-1 beta treatment. Gene expression profiling was performed with a GeneChip. Changes in gene expression were determined by comparing IL-1 beta-treated cells with untreated cells.RESULTS: A total of 121 genes showed a greater than threefold difference in average intensity between untreated and IL-1 beta-treated synovial fibroblasts in five experiments. Five chemokines were among the 10 most upregulated genes, and the most upregulated gene was CCL20. The 121 IL-1 beta-responsive genes included 12 chemokines whose mRNA levels were confirmed by real-time PCR.CONCLUSION: These data should provided useful information about the pathologic conditions of TMJ, especially in support of diagnosis and therapeutic approaches to TMJ.