Coupling Variation Induced Ultrasensitive Label-Free Biosensing by Using Single Mode Coupled Microcavity Laser
Coupling Variation Induced Ultrasensitive Label-Free Biosensing by Using Single Mode Coupled Microcavity Laser
复制标题
使用单模耦合微腔激光器耦合变化诱导超灵敏无标记生物传感
DOI：
10.1021/ja9055728
复制
发表时间：
2009-11-25
影响因子：
15
通讯作者：
Xu, Lei
中科院分区：
文献类型：
--
作者：
Li, Hao;Shang, Lei;Xu, Lei
利用单模耦合微腔激光器，实验实现了一种新型的无标记光学生物传感方案。我们证明，由于生物样品的存在，耦合系数的微小变化导致灵敏的单模激光跳跃到距离原始模式波长几nm的新频率。通过监测跳跃激光模强度的出现，获得了牛血清白蛋白(BSA)的最低检测浓度，接近80pg/mL，与Q>；10(7)的被动微腔传感器的检测下限相当，但实验装置要简单得多。单频耦合腔激光器结合了跳模和移模技术，可提供从pg/m L(跳模极限)到m g/m L(模移极限)的探测范围。结果表明，利用耦合光学微腔实现超灵敏光学传感是可能的。
A novel label-free optical biosensing scheme by using a single mode coupled microcavity laser is experimentally realized. We demonstrate that a slight change of the coupling coefficient due to the existence of biosamples leads to sensitive single mode laser hopping to a new frequency that is several nm away from the original mode wavelength. By monitoring the emergence of the hopped laser mode intensity, the lowest detectable concentration of bovine serum albumin (BSA) of similar to 80 pg/mL was obtained, which is comparable to the detection limit of a passive microcavity sensor with Q > 10(7), but with a much simplified experimental setup. With the mode hopping and mode shift combined, the single frequency coupled cavity laser provides a detection range from pg/mL (Limit of mode hopping) to mu g/mL (Limit of mode shift). Our results show the possibility of using a coupled optical microcavity to achieve ultrasensitive optical sensing.