Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs).
Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs).
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DOI：
10.1186/s12953-017-0123-3
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发表时间：
2016
期刊：
影响因子：
2
通讯作者：
Lee JS
中科院分区：
文献类型：
--
作者：
Murale DP;Hong SC;Haque MM;Lee JS
蛋白质-蛋白质相互作用（PPI）触发了广泛的生物信号通路，这些通路对于生物医学研究和药物发现至关重要。各种技术已被用于研究特定的蛋白质，包括亲和层析，基于活性的探针，基于亲和力的探针和光亲和标记（PAL）。PAL已成为研究PPI的最有力的策略之一。PAL中使用传统的光交联剂，包括二苯甲酮、芳基叠氮化物和二氮丙啶。在光照射时，这些光交联剂（Pls）产生与相邻分子反应的高度反应性物质，导致直接共价修饰。本文介绍了近年来PAL在PPI化学蛋白质组学研究中的应用。
Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.