A Pseudomonas Putida Strain Able To Degrade M-Toluate in the Presence of 3-Chlorocatechol
A Pseudomonas Putida Strain Able To Degrade M-Toluate in the Presence of 3-Chlorocatechol
复制标题
3-氯儿茶酚存在下能够降解间甲苯甲酸的恶臭假单胞菌菌株
DOI：
10.1038/nbt0391-296
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Harayama
中科院分区：
文献类型：
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作者：
A. Wasserfallen;M. Rekik;S. Harayama
3-氯儿茶酚抑制携带TOL质粒的恶臭假单胞菌在甲苯甲酸上的生长。来自 aP。 putidastrain携带负责氧化苯甲酸甲酯的克隆TOL基因，我们分离出一种能够在3-氯苯甲酸存在下生长对甲苯甲酸的衍生物。点突变 inxylE（儿茶酚 2,3-双加氧酶的结构基因）产生缬氨酸 291 取代异亮氨酸 291，导致了分离株的生长特性。与野生型酶相比，突变型儿茶酚2,3-双加氧酶(i)对3-氯儿茶酚的亲和力降低，(ii)对3-甲基儿茶酚的亲和力增加，以及(iii)氧化4-乙基儿茶酚的效率增加。该菌株可用于分解含有3-氯苯甲酸酯和间甲苯甲酸酯的工业废物中的间甲苯甲酸酯。
Growth onm-toluate ofPseudomonas putidacarrying the TOL plasmid is inhibited by 3-chlorocatechol. From aP. putidastrain, carrying cloned TOL genes responsible for the oxidation of methylbenzoates, we isolated a derivative able to grow onm-toluate in the presence of 3-chlorobenzoate. A point mutation inxylE, the structural gene for catechol 2,3-dioxygenase, which produced the substitution of valine-291 for isoleucine-291, was responsible for the growth characteristics of the isolate. The mutant catechol 2,3-dioxygenase has (i) decreased affinity for 3-chlorocatechol, (ii) increased affinity for 3-methylcatechol, and (iii) increased efficiency in oxidizing 4-ethylcatechol, compared to the wild-type enzyme. This strain may be useful for the decomposition ofm-toluate in industrial waste containing both 3-chlorobenzoate andm-toluate.