Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation
Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation
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DOI：
10.1128/mbio.02876-18
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发表时间：
2019-01-01
期刊：
影响因子：
6.4
通讯作者：
Liu, Shuang-Jiang
中科院分区：
文献类型：
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作者：
Huang, Zhou;Wang, Yun-Hao;Liu, Shuang-Jiang
复杂的化学感觉系统控制着细菌的多种生物功能，如趋化性、基因调控和细胞周期进程。许多物种每个基因组包含不止一个化学感觉系统，但对它们之间潜在的相互作用知之甚少。在这项研究中，我们揭示了在睾酮单胞菌中调节趋化性和生物膜形成的两种化学感觉通路之间的串扰。我们证明了一些控制趋化性的化学感受器也有助于生物膜的形成，这些化学感受器可以物理地与这两种途径的成分相互作用。最后，我们发现趋化性组氨酸激酶CheA不仅可以磷酸化其同源反应调节因子CheY2，还可以磷酸化介导生物膜形成途径的反应调节因子之一FlmD。磷酸化基团从CheA到CheY2的转移要比从CheA到FlmD的转移快得多，这与趋化是一个快速的反应而生物膜的形成是一个慢得多的发育过程是一致的。我们提出化学感觉通路之间的串扰可能在细菌复杂行为的协调中起作用。在许多细菌中，两种或两种以上的同源化学感觉通路控制着一些细胞功能，如运动和基因调节，以响应细胞微环境的变化。人们对信号转导系统之间的串扰知之甚少；虽然它通常被认为是不希望的，但在某些情况下，它可能有利于复杂行为的协同调节。我们证明了来自控制运动途径的几个受体可以与控制生物膜形成途径的下游组分物理相互作用。我们进一步表明，来自控制运动途径的激酶也可以磷酸化来自控制生物膜形成途径的反应调节因子。我们认为两种化学感觉通路之间的串话可能参与了两种类型的细胞行为-趋化性和生物膜形成的协调。
Complex chemosensory systems control multiple biological functions in bacteria, such as chemotaxis, gene regulation, and cell cycle progression. Many species contain more than one chemosensory system per genome, but little is known about their potential interplay. In this study, we reveal cross talk between two chemosensory pathways that modulate chemotaxis and biofilm formation in Comamonas testosteroni. We demonstrate that some chemoreceptors that govern chemotaxis also contribute to biofilm formation and these chemoreceptors can physically interact with components of both pathways. Finally, we show that the chemotaxis histidine kinase CheA can phosphorylate not only its cognate response regulator CheY2 but also one of the response regulators from the pathway mediating biofilm formation, FlmD. The phosphoryl group transfer from CheA to CheY2 is much faster than that from CheA to FlmD, which is consistent with chemotaxis being a fast response and biofilm formation being a much slower developmental process. We propose that cross talk between chemosensory pathways may play a role in coordination of complex behaviors in bacteria.IMPORTANCE In many bacteria, two or more homologous chemosensory pathways control several cellular functions, such as motility and gene regulation, in response to changes in the cell's microenvironment. Cross talk between signal transduction systems is poorly understood; while generally it is considered to be undesired, in some instances it might be beneficial for coregulation of complex behaviors. We demonstrate that several receptors from the pathway controlling motility can physically interact with downstream components of the pathway controlling biofilm formation. We further show that a kinase from the pathway controlling motility can also phosphorylate a response regulator from the pathway controlling biofilm formation. We propose that cross talk between two chemosensory pathways might be involved in coordination of two types of cell behavior-chemotaxis and biofilm formation.