Variation in cadmium accumulation and speciation within the same population of the hyperaccumulator Noccaea caerulescens grown in a moderately contaminated soil
Variation in cadmium accumulation and speciation within the same population of the hyperaccumulator Noccaea caerulescens grown in a moderately contaminated soil
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DOI：
10.1007/s11104-022-05373-w
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发表时间：
2022-03
期刊：
影响因子：
4.9
通讯作者：
Jiali Yan;Zhong Tang;M. Fischel;Peng Wang;Matthew G. Siebecker;Mark G. M. Aarts;D. Sparks;F. Zhao
中科院分区：
文献类型：
--
作者：
Jiali Yan;Zhong Tang;M. Fischel;Peng Wang;Matthew G. Siebecker;Mark G. M. Aarts;D. Sparks;F. Zhao
背景与目的植物提取是修复重金属污染土壤的一种生态友好的方法。目的筛选镉(Cd)植物提取效率较高的褐藻品系，并研究Cd高、低积累品系叶片中Cd形态和分布的差异。方法采用盆栽试验，在中度Cd污染土壤(2.1mgCd kg− 1)上，对从Cd超积累炉甘石种群中单粒下降产生的29个褐藻品系的生物产量和Cd的生物积累能力进行了评价。结果6个月后，蓝藻生物量最大，达到5.0 ± 3.3g(D.W.pot− 1)。地上部Cd浓度变化范围为85~2 0 3m g kg− 1，最有效的品系去除0.64m g盆栽− 1，使土壤全Cd降低30%。基于同步加速器的X射线吸收光谱表明，Cd的主要物种是Cd-硫醇配合物。高、低Cd积累系的叶片中分别存在Cd-羧基和Cd-植酸/磷酸盐。显微X射线荧光显微镜显示Cd主要集中在叶脉中。结论在同一种炉甘石生态型中，不同品系的生物量和Cd的积累量存在较大差异，Cd-硫醇络合物在中度Cd污染的水稻土中对Cd的解毒作用最大。这些结果为选育高Cd积累量的蓝藻品种提供了生理基础。
Background and aimsPhytoextraction is an eco-friendly approach for remediation of heavy metal contaminated soil. The aim is to screenNoccaea caerulescenslines with higher cadmium (Cd) phytoextraction efficiency and investigate differences in Cd species and distribution in the leaves of high and low Cd accumulating lines.MethodsBiomass production and Cd bioaccumulation capacities of 29Noccaea caerulescenslines, generated through single-seed-descent from a Cd hyperaccumulating calamine population, were assessed in a pot experiment with a moderately Cd contaminated soil (2.1 mg Cd kg− 1). Synchrotron-based techniques were employed to identify and characterize Cd speciation and distribution inNoccaea caerulescensleaves.ResultsThe largest biomass ofNoccaea caerulescensreached 5.0 ± 3.3 g (D. W. pot− 1) after 6 months growth. The Cd concentrations in shoots varied from 85 to 203 mg kg− 1. The most efficient line removed 0.64 mg Cd pot− 1and lowered the total Cd in soil by 30%. Synchrotron-based X-ray absorption spectroscopy showed that the dominant Cd species was Cd-thiol complexes. Cadmium-carboxyl and Cd-phytate/phosphate were present in the leaves of high and low Cd accumulating lines, respectively. Micro X-ray fluorescence microscopy showed cadmium was concentrated in leaf veins.ConclusionsThere are wide variations including both biomass production and Cd accumulation capacity among different lines within the same calamine ecotype ofNoccaea caerulescens.Cadmium-thiol complexes play the most important role in Cd detoxification in leaves ofNoccaea caerulescensgrown in moderately Cd contaminated paddy soil. These findings provide a physiological basis for breeding high Cd accumulation varieties ofNoccaea caerulescens.