MOLECULAR ANALYSIS BY PULSED-FIELD GEL-ELECTROPHORESIS OF PENICILLIN-RESISTANT STREPTOCOCCUS-PNEUMONIAE FROM TOULOUSE, FRANCE
MOLECULAR ANALYSIS BY PULSED-FIELD GEL-ELECTROPHORESIS OF PENICILLIN-RESISTANT STREPTOCOCCUS-PNEUMONIAE FROM TOULOUSE, FRANCE
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DOI：
10.1007/bf02113426
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发表时间：
1995-06-01
影响因子：
4.5
通讯作者：
FAUCON, G
中科院分区：
文献类型：
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作者：
LEFEVRE, JC;BERTRAND, MA;FAUCON, G
对1991年至1993年在法国图卢兹的一家大型医院分离的28株青霉素耐药肺炎链球菌的样本进行了基因组DNA的脉冲场凝胶电泳表征。还包括来自图卢兹的6株青霉素敏感临床分离株和来自世界不同地区的12株青霉素耐药菌株。用限制性内切酶Apal和Smal消化完整的染色体，并通过场反转凝胶电泳解析片段。从图卢兹分离的青霉素耐药菌株中可以识别出7种主要的模式类型。这些分离株中的9个可以被分配到两个克隆，这两个克隆也在西班牙发现，并与血清型6B和9V相关。在南非和西班牙分离出第三个克隆，其中含有se重组23F分离株。场反转凝胶电泳得到的配置文件表明，15的16个青霉素耐药血清组23分离图卢兹属于相同的西班牙23 F克隆。除9V株外，青霉素敏感株与同一血清型的耐药株的分子检测谱不同。这些数据强调了来自西班牙的耐药克隆在法国出现青霉素耐药肺炎球菌的地理传播的重要性。
A sample of 28 penicillin-resistant Streptococcus pneumoniae strains isolated between 1991 and 1993 in a large hospital in Toulouse, France, was characterized by pulsed-field gel electrophoresis of genomic DNA. Also included were 6 penicillin-susceptible clinical isolates from Toulouse and 12 penicillin-resistant strains from different parts of the world. The restriction endonucleases Apal and Smal were used to digest intact chromosomes, and the fragments were resolved by field-inversion gel electrophoresis. Seven major pattern types could be recognized among the penicillin-resistant isolates from Toulouse. Nine of these isolates could be assigned to two clones that were also found in Spain and were associated with serotypes 6B and 9V. A third clone was isolated in South Africa and in Spain and contained se retype 23F isolates. The profiles obtained by field-inversion gel electrophoresis suggested that 15 of the 16 penicillin-resistant serogroup 23 isolates from Toulouse belonged to the same Spanish 23F clone. The molecular test profiles of penicillin-susceptible strains differed from those of resistant strains of the same serotype except those of 9V strains. These data underline the importance of the geographic spread of resistant clones from Spain in the emergence of penicillin-resistant pneumococci in France.