Reproductive toxicity of nanoscale graphene oxide in male mice
Reproductive toxicity of nanoscale graphene oxide in male mice
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纳米氧化石墨烯对雄性小鼠的生殖毒性
DOI：
10.3109/17435390.2014.893380
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发表时间：
2015-02-01
期刊：
影响因子：
5
通讯作者：
Chu, Maoquan
中科院分区：
文献类型：
--
作者：
Liang, Shanlu;Xu, Shun;Chu, Maoquan
摘要 在过去的几年里，人们进行了大量的工作来探索纳米石墨烯及其衍生物的生物医学应用和毒性。然而，这些碳纳米材料的生殖毒性却很少被研究。在这项研究中，我们利用小鼠模型报告了纳米氧化石墨烯 (GO) 的雄性生殖毒性。结果表明，尾静脉注射高剂量GO（25mg/kg小鼠）的成年雄性小鼠表现出正常的性激素分泌，并保留了正常的生殖活动。所有未经治疗的雌性小鼠与经过 GO 治疗的雄性小鼠交配都可以产下健康的幼崽。 GO处理组和对照组之间的幼仔数量、性别比例、体重、幼仔存活率或幼仔生长随时间的变化没有显着差异。此外，当这些接受 GO 治疗的雄性小鼠与未经治疗的雌性小鼠生活在一起时，它们可以产下第二、第三、第四甚至第五窝健康的后代。睾丸和附睾组织学以及几种重要附睾酶（包括α-葡萄糖苷酶、乳酸脱氢酶、谷胱甘肽过氧化物酶和酸性磷酸酶）的活性不受GO处理的影响。此外，通过腹内注射的高剂量率GO（雄性小鼠总计300mg/kg，每24小时60mg/kg，持续5天）没有看到损害作用。因此，GO 对雄性生殖表现出非常低或几乎没有毒性。这项工作将极大地促进未来 GO 纳米片在体内生物医学应用的研究。
Abstract In the past few years, much work has been performed to explore the biomedical applications and toxicity of nano-graphene and its derivatives. However, the reproductive toxicity of those carbon nanomaterials has been rarely studied. In this study, we report on the male reproductive toxicity of nanoscale graphene oxide (GO) using a mouse model. The results showed that the adult male mice injected with high dosages of GO (25 mg/kg mouse) via the tail vein exhibited normal sex hormone secretion and retained normal reproductive activity. All untreated female mice mated with the GO-treated male mice could produce healthy pups. There were no significant differences in pup numbers, sex ratio, weights, pup survival rates or pup growth over time between the GO-treated and control groups. Furthermore, these GO-treated male mice could produce a second, third, fourth and even fifth litter of healthy offspring when they lived with the untreated female mice. The testicular and epididymal histology as well as the activities of several important epididymal enzymes including α-glucosidase, lactate dehydrogenase, glutathione peroxidase and acid phosphatase were not affected by GO treatment. In addition, no damaging effects were seen at high dose rates of GO (total 300 mg/kg male mouse, 60 mg/kg every 24 h for 5 days) via intra-abdominal injection. Thus, GO showed very low or nearly no toxicity for male reproduction. This work will greatly enable future investigations of GO nanosheets for in vivo biomedical applications.