Repository Citation This Work Is Licensed under a Creative Commons Attribution 4.0 License
Repository Citation This Work Is Licensed under a Creative Commons Attribution 4.0 License
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通讯作者：
Thomas Musich;Olivia O 'connell;Maria Paz Gonzalez-Perez;Olivia O 'connell;M. Gonzalez-Perez;Paz
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作者：
Thomas Musich;Olivia O 'connell;Maria Paz Gonzalez-Perez;Olivia O 'connell;M. Gonzalez-Perez;Paz
HIV-1非巨噬细胞亲和性R5包膜糖蛋白并不比高度适应巨噬细胞的包膜更倾向于进入初级CD4+T细胞。HIV-1非巨噬细胞嗜性的R5包膜糖蛋白并不比高度适合巨噬细胞的包膜更倾向于进入原始T细胞。这是一篇开放获取的文章，根据知识共享署名许可证(http://creativecommons.org/licenses/by/4.0)，)的条款分发，允许在任何介质中不受限制地使用、分发和复制，只要原始作品得到适当的信用。知识共享公共领域奉献豁免HIV-1非巨噬细胞嗜性R5囊膜糖蛋白并不比高度适应巨噬细胞的囊膜更倾向于进入原代CD4+T细胞。背景：非嗜Mac HIV-1R5病毒主要传播并在免疫组织中存活，即使在脑中携带高度嗜巨噬细胞变异体的艾滋病患者中也是如此。与高度嗜Mac的R5包膜相比，非嗜Mac的R5包膜(Env)需要高水平的CD4才能感染，后者更有效地与CD4相互作用，并介导表达低CD4的巨噬细胞的感染。非嗜Mac的R5在传播和免疫组织中主要针对T细胞，在那里它们必须比嗜Mac的变体更具竞争力。在这里，我们研究了携带传播的/方正(T/F)、早期和晚期疾病的非嗜Mac R5包膜的Env+伪病毒是否比那些高度嗜Mac的Env更有效地介导了对CD4+T细胞的感染。结果：高度嗜Mac的EVS对原代T细胞、Jurkat/CCR5细胞、髓系树突状细胞、巨噬细胞和HeLa TZM-bl细胞的感染力最高，但对巨噬细胞的感染力最强。所有EVS介导的原代T细胞感染率均较低。然而，通过增加DEAE葡聚糖和Spin ococation对病毒的附着，大大增强了T细胞的感染，这对三个Env+病毒组的增强程度相似。树突状细胞捕获病毒和反式感染也大大增强了原代T细胞的感染。在转染性试验中，非嗜Mac R5env优先增强，晚期疾病所介导的T细胞感染水平与嗜Mac env所介导的水平相当。
HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages "HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages" (2015). HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages Comments This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages Abstract Background: Non-mac-tropic HIV-1 R5 viruses are predominantly transmitted and persist in immune tissue even in AIDS patients who carry highly mac-tropic variants in the brain. Non-mac-tropic R5 envelopes (Envs) require high CD4 levels for infection contrasting with highly mac-tropic Envs, which interact more efficiently with CD4 and mediate infection of macrophages that express low CD4. Non-mac-tropic R5 Envs predominantly target T-cells during transmission and in immune tissue where they must outcompete mac-tropic variants. Here, we investigated whether Env+ pseudoviruses bearing transmitted/founder (T/F), early and late disease non-mac-tropic R5 envelopes mediated more efficient infection of CD4+ T-cells compared to those with highly mac-tropic Envs. Results: Highly mac-tropic Envs mediated highest infectivity for primary T-cells, Jurkat/CCR5 cells, myeloid dendritic cells, macrophages, and HeLa TZM-bl cells, although this was most dramatic on macrophages. Infection of primary T-cells mediated by all Envs was low. However, infection of T-cells was greatly enhanced by increasing virus attachment with DEAE dextran and spinoculation, which enhanced the three Env+ virus groups to similar extents. Dendritic cell capture of viruses and trans-infection also greatly enhanced infection of primary T-cells. In trans-infection assays, non-mac-tropic R5 Envs were preferentially enhanced and those from late disease mediated levels of T-cell infection that were equivalent to those mediated by mac-tropic Envs.