Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells.
Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells.
复制标题
中间多能干细胞的衍生和原始生殖细胞诱导。
DOI：
10.1007/978-1-0716-3259-8_16
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Wu,Jun
中科院分区：
文献类型：
--
作者：
Yu,Leqian;Ballard,Emily;PinzonArteaga,CarlosA;Wu,Jun
动态多能干细胞（PSC）状态是体内多能连续体的体外适应。先前的研究已经产生了许多具有不同特性的PSC。通过调节FGF、TGF-β和WNT通路，我们获得了可直接体外诱导原始生殖细胞样细胞（PGC-LC）的中间体PSC（FTW-PSC），本文描述了小鼠和人FTW-PSC的衍生和维持方法，以及FTW-PSC诱导PGC-LC的方法。
Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of the pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro.Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.