Red Ginseng Inhibits Scratching Behavior Associated With Atopic Dermatitis in Experimental Animal Models
Red Ginseng Inhibits Scratching Behavior Associated With Atopic Dermatitis in Experimental Animal Models
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DOI：
10.1254/jphs.11182fp
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发表时间：
2012-03-01
影响因子：
3.5
通讯作者：
Iwao, Hiroshi
中科院分区：
文献类型：
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作者：
Samukawa, Keiichi;Izumi, Yasukatsu;Iwao, Hiroshi
瘙痒是特应性皮炎患者较难治疗的严重症状。红参（RG）是一种天然药物，具有抗炎等多种生物活性。本研究探讨了RGE提取物对小鼠实验性特应性皮炎的治疗作用及其机制。首先评价RGE对血管通透性和瘙痒的影响。用RGE和抗组胺药物苯海拉明（苯海拉明）涂抹组胺诱导的通透性和瘙痒明显抑制。接下来，我们评估了局部RGE对特应性皮炎小鼠模型的治疗效果。将2,4-二硝基氟苯（DNFB）丙酮溶液反复应用于小鼠耳内，引起小鼠皮炎。比较他克莫司（一种钙调磷酸酶阻滞剂）、地塞米松（一种皮质类固醇）和RGE对皮炎和相关抓伤行为的影响。反复应用DNFB引起频繁抓痒行为和耳部肿胀。他克莫司、地塞米松和RGE局部治疗DNFB前8天可显著抑制耳肿胀。他克莫司和RGE显著抑制抓痕行为，而地塞米松没有。他克莫司和RGE可显著减弱dnfb诱导的神经生长因子表达和神经纤维延伸，而地塞米松则无明显作用。RGE可能具有治疗特应性皮炎的潜力。
Pruritus is a severe symptom that is difficult to treat in atopic dermatitis patients. Red ginseng (RG), a natural medicine, has various biological activities such as anti-inflammatory effects. In this study, we examined the efficacy of RG extract (RGE) and its mechanism on experimental atopic dermatitis in mice. The effects of RGE on vascular permeability and itching were first evaluated. Histamine-induced permeability and itching were significantly inhibited by embrocation with RGE as well as diphenhydramine, an antihistamine drug. Next, we assessed the therapeutic effect of topical RGE in a mouse model of atopic dermatitis. Dermatitis was induced by repeated application of 2,4-dinitrofluorobenzene (DNFB) acetone solution to the mouse ear. The effects of tacrolimus (a calcineurin blocker), dexamethasone (a corticosteroid), and RGE on dermatitis and associated scratching behavior were compared. Repeated DNFB application caused frequent scratching behaviors and ear swelling. Topical treatment with tacrolimus, dexamethasone, and RGE for 8 days before the final challenge with DNFB significantly inhibited ear swelling. Tacrolimus and RGE significantly inhibited scratching behavior, whereas dexamethasone failed to do so. DNFB-induced nerve growth factor expression and nerve fiber extension were significantly attenuated by tacrolimus and RGE, but not by dexamethasone. RGE may have the potential for treatment of atopic dermatitis.