Mitotic activity in the epithelium of the epididymis in young and old adult rats.
Mitotic activity in the epithelium of the epididymis in young and old adult rats.
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DOI：
10.1093/biolreprod/3.3.283
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发表时间：
1970-12
影响因子：
3.6
通讯作者：
Y. Clermont;J. Flannery
中科院分区：
文献类型：
--
作者：
Y. Clermont;J. Flannery
通过注射3h -胸腺嘧啶动物的标记指数和有丝分裂指数来评估附睾上皮细胞的增殖活性，分别在2.5个月、4个月和12个月的三组成年白化大鼠中进行。2.5月龄大鼠的主细胞和基底细胞标记指数较高（整个附睾的主细胞标记指数为2.2%，基底细胞标记指数为1.4%），而4月龄和12月龄大鼠的主细胞和基底细胞标记指数较低（低于0.6%）。在2.5个月大的动物中，3h -胸腺嘧啶标记的细胞此后在上皮内停留了很长一段时间（实验终止时长达50天）。非立体纤毛的窄柱状细胞和光细胞没有明显的标记，也没有细胞丢失的迹象。有丝分裂指数在2.5月龄大鼠中明显，但在老年动物中不明显。注射秋水仙碱的4月龄动物的有丝分裂指数与未注射秋水仙碱的动物相似。这些数据表明，在性成熟但年轻的成年大鼠中，附睾仍在生长。在老年大鼠中，附睾继续生长，但速度明显较低。在这些动物中，附睾上皮细胞的低有丝分裂指数和标记指数将该细胞群划分为扩张而不是更新的细胞群。因此，成年大鼠的附睾中没有全息分泌。
The proliferative activity of epithelial cells lining the epididymis as judged from labeling indices in3H-thymidine-injected animals and from mitotic indices was evaluated in three groups of adult albino rats, respectively, aged 2.5, 4, and 12 months.The labeling indices of principal and basal cells were relatively high in 2.5-month-old rats (2.2% of principal cells and 1.4% of basal cells for the whole epididymis) but low (below 0.6%) in 4-and 12-month-old animals. In the 2.5-month-old animals the cells labeled with3H-thymidine stayed within the epithelium for a long period of time thereafter (up to 50 days when the experiment was terminated). There was no appreciable labeling of the nonstereociliated narrow columnar and light cells and no sign of cell loss. Mitotic indices were appreciable in 2.5-month-old rats but insignificant in older animals. Mitotic indices obtained from 4-month-old animals injected with colchicine were similar to that of noncolchicine-injected animals. These data indicated that in sexually mature but young adult rats growth was still taking place in the epididymis. In older rats growth of the epididymis continued but at a considerably lower rate. In these animals the low mitotic and labeling indices of the epididymal epithelial cells classified this cell population amongst the expanding rather than amongst the renewing ones. No holocrine secretion must therefore take place in the epididymis of adult rats.