Diurnal variation in 5-hydroxyindole-acetic acid output in the suprachiasmatic region of the Siberian hamster assessed by in vivo microdialysis: evidence for nocturnal activation of serotonin release.
Diurnal variation in 5-hydroxyindole-acetic acid output in the suprachiasmatic region of the Siberian hamster assessed by in vivo microdialysis: evidence for nocturnal activation of serotonin release.
复制标题
通过体内微透析评估西伯利亚仓鼠视交叉上区域 5-羟基吲哚-乙酸输出的昼夜变化：夜间激活血清素释放的证据。
DOI：
10.1159/000126277
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发表时间：
1992
影响因子：
4.1
通讯作者：
M. D. de Vries
中科院分区：
文献类型：
--
作者：
J. Glass;W. Randolph;S. Ferreira;M. Rea;U. E. Hauser;J. Blank;M. D. de Vries
用体内脑微透析方法研究了16L:8D条件下自由活动的雄性西伯利亚仓鼠视交叉上核（SCN）区域5-羟基吲哚-乙酸（5-HIAA）的每日释放规律。细胞外5-HIAA浓度的日变化明显，在熄灯后2-3小时达到峰值（147 +/- 5%,p < 0.05）。在夜间，下丘脑后部和视前区细胞外5-HIAA的升高幅度较小（分别为每日平均值的128 +/- 4%和123 +/- 8%；与白天平均水平相比，p < 0.05）。色氨酸负载使SCN微透析液中的5- hiaa增加了44 +/- 6%，局部灌注河豚毒素（TTX； 5微米）增强了这种反应。局部施用KCl （150 mM）或veratridine（100微米）分别使5- hiaa降低62% +/- 5%或49% +/- 11%。特异性钙通道阻滞剂对KCl的作用无显著影响。在暗期灌注TTX可显著降低SCN 5-HIAA，但在光照期效果不明显（分别抑制42 +/- 8%和12 +/- 5%，p < 0.01）。在灌注液中加入3微米的血清素可显著刺激5-HIAA的输出。该处理在暗期比光照期更能增加5-HIAA的释放（分别为61 +/- 8%和25 +/- 5%，p < 0.01）。（摘要删节250字）
In vivo brain microdialysis was used to characterize the daily pattern of 5-hydroxyindole-acetic acid (5-HIAA) release in the region of the suprachiasmatic nuclei (SCN) in freely behaving male Siberian hamsters housed under 16L:8D. A marked diurnal variation in the concentration of extracellular 5-HIAA was apparent, with peak levels (147 +/- 5% of the daily mean; p < 0.05) occurring 2-3 h after lights-off. Smaller nocturnal rises in extracellular 5-HIAA were observed in the posterior hypothalamus and preoptic area (128 +/- 4 and 123 +/- 8% of the daily mean, respectively; both p < 0.05 vs. average daytime levels). Tryptophan loading increased 5-HIAA in SCN microdialysates by 44 +/- 6%, and this response was enhanced by localized perfusion with tetrodotoxin (TTX; 5 microM). Localized applications of KCl (150 mM) or veratridine (100 microM) decreased 5-HIAA by 62 +/- 5 or 49 +/- 11%, respectively. The effect of KCl was not significantly affected by specific calcium channel blockers. Perfusion with TTX markedly decreased SCN 5-HIAA during the dark phase, but had little effect during the light phase (42 +/- 8 vs. 12 +/- 5% suppression, respectively; p < 0.01). Addition of serotonin (3 microM) to the perfusate significantly stimulated 5-HIAA output. This treatment increased the release of 5-HIAA more during the dark than during the light phase (61 +/- 8 vs. 25 +/- 5%, respectively; p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)