Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: Effect of surface modification on cell attachment and function
Biodegradable PLGA microcarriers for injectable delivery of chondrocytes: Effect of surface modification on cell attachment and function
复制标题
DOI：
10.1021/bp0496981
复制
发表时间：
2004-11-01
影响因子：
2.9
通讯作者：
Park, TG
中科院分区：
文献类型：
--
作者：
Chun, KW;Yoo, HS;Park, TG
采用油/水乳化溶剂挥发法制备了聚(D，L-乳酸-乙醇酸共聚)微球，并将其作为细胞给药的注射微载体。制备了具有疏水、带负电和带正电三种不同表面的PLGA微球。用末端封端的PLGA聚合物和未封端的PLGA聚合物分别制备了疏水和带负电荷的PLGA微球。以PLGA-g-聚(L-赖氨酸)接枝共聚物为表面修饰剂，与PLGA共混制得带正电的PLGA微球。在血清条件下，将牛软骨细胞培养在三种PLGA微球上，以比较评估细胞附着、细胞增殖和细胞功能的表面性质。与疏水和带负电荷的微球相比，带正电的PLGA微球显示出最高的细胞附着、生长和功能。表面修饰的PLGA微球有可能被用作细胞注入组织缺损区的可注射递送系统。
Poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres were prepared by an oil/water emulsion solvent evaporation method to use as an injectable microcarrier for cell delivery. Three different kinds of PLGA microspheres having hydrophobic, negatively charged, and positively charged surfaces were prepared. Hydrophobic and negatively charged PLGA microspheres were prepared by using terminally capped and uncapped PLGA polymer, respectively. Positively charged PLGA microspheres were prepared by blending PLGA with PLGA-g-poly(L-lysine) graft copolymer as a surface modifying agent. Bovine chondrocytes were cultured on the three PLGA microspheres under serum conditions to comparatively evaluate cell attachment, cell proliferation, and cell function with respect to surface properties. Positively charged PLGA microspheres showed the highest cell attachment, growth, and function compared to hydrophobic and negatively charged microspheres. Surface-modified PLGA microspheres can potentially be used as an injectable delivery system for cells into a tissue defect site.