Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry
Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry
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DOI：
10.1369/jhc.6a7156.2007
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Dickson, Dennis W.
中科院分区：
文献类型：
--
作者：
Ahmed, Zeshan;Shaw, Gerry;Dickson, Dennis W.
本研究确定肌动蛋白结合蛋白，冠蛋白-la，作为一种新的和有效的免疫组化标记小胶质细胞在细胞培养和甲醛固定，石蜡包埋组织。冠蛋白-la的抗体有效地免疫染色人、猴、马、大鼠和小鼠组织中的小胶质细胞，甚至在长时间储存的组织中。冠状蛋白-LA-免疫反应细胞作为小胶质细胞的身份确认使用双重免疫标记与细胞类型特异性标记，以及通过形态学特征和免疫反应细胞的分布。另一种肌动蛋白结合蛋白IBA-1也具有这些特性。与IBA-1不同的是，冠蛋白-1a免疫反应性也在淋巴细胞和某些其他造血细胞中检测到。结果表明，冠蛋白-1 α和IBA-1都是小胶质细胞的稳健标志物，可用于人类和动物的常规处理组织。由于冠蛋白-la和IBA-1都是肌动蛋白结合蛋白，在膜细胞骨架的重排中发挥作用，这表明这些蛋白质对小胶质细胞的动态特性至关重要。
This study identifies the actin-binding protein, coronin-la, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-la effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-la-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1 a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1 a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-la and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.