A NEW MODEL OF EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION IN THE RAT
A NEW MODEL OF EXPERIMENTAL CHOROIDAL NEOVASCULARIZATION IN THE RAT
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DOI：
10.1001/archopht.1989.01070010270035
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发表时间：
1989-02-01
影响因子：
--
通讯作者：
DESTRO, M
中科院分区：
文献类型：
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作者：
DOBI, ET;PULIAFITO, CA;DESTRO, M
氪激光光凝诱导大鼠眼脉络膜新生血管形成。每周通过检眼镜检查、眼底照相和荧光素血管造影对病变进行研究。通过光学显微镜和透射电子显微镜对病变进行连续切片来提供形态学相关性。此外，还制备了血管铸型用于扫描电子显微镜检查。 42 个病变中的 25 个（60%）发生了脉络膜新生血管形成，毛细血管通过布鲁赫膜破裂生长即可证明。此外，通过荧光素血管造影研究的 86 个病变中，有 24 个（28%）显示渗漏。这项研究提供了大鼠实验性脉络膜新生血管形成的最完整的血管造影、组织学和超微结构文件。该模型可用于脉络膜血管生成及其通过药物治疗的调节的体内研究。
Choroidal neovascularization in rat eyes was induced by krypton laser photocoagulation. Lesions were studied weekly by ophthalmoscopy, fundus photography, and fluorescein angiography. Morphologic correlation was provided by serial sectioning of lesions for light and transmission electron microscopy. In addition, vascular casts were prepared for scanning electron microscopy. Choroidal neovascularization occurred in 25 (60%) of 42 lesions, as evidenced by growth of capillaries through breaks in Bruch''s membrane. In addition, 24 (28%) of 86 lesions studied by fluorescein angiography demonstrated leakage. This study provides the most complete angiographic, histologic, and ultrastructural documentation of experimental choroidal neovascularization in the rat. This model may be useful for in vivo studies of choroidal angiogenesis and its modulation via drug therapy.