Effects of acute sleep loss on diurnal plasma dynamics of CNS health biomarkers in young men
Effects of acute sleep loss on diurnal plasma dynamics of CNS health biomarkers in young men
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DOI：
10.1212/wnl.0000000000008866
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发表时间：
2020-03-17
期刊：
影响因子：
9.9
通讯作者：
Cedernaes, Jonathan
中科院分区：
文献类型：
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作者：
Benedict, Christian;Blennow, Kaj;Cedernaes, Jonathan
睡眠中断会增加CSF中tau和β-淀粉样蛋白（A β）的水平，并与阿尔茨海默病（AD）的风险增加有关。我们的目的是确定急性睡眠不足是否会改变昼夜配置文件的血浆为基础的AD-相关的biomarkers.MethodsIn一个2条件交叉研究，15名健康的年轻男子参加了2个标准化的久坐不动的实验室条件随机顺序：正常睡眠与夜间睡眠不足。在每次干预前的晚上和干预后的早晨，使用超灵敏单分子阵列分析或ELISA评估总tau（t-tau）、A β 40、A β 42、神经丝轻链（NfL）和胶质细胞酸性蛋白（GFAP）的血浆水平。（+17.2%），与正常睡眠（+1.8%）相比，t-tau的夜间与早晨比率增加（p = 0.035）。在睡眠条件下，A β 40、A β 42、NfL或GFAP水平没有变化（所有p > 0.10）。AD风险基因型rs 4420638与睡眠丧失相关的血浆A β 40或A β 42水平的昼夜变化无显著相互作用（p > 0.10）。A β 42（-17.1%）和GFAP（-12.1%）的血浆水平在不同条件下从晚上到早上都有所下降（p < 0.05）。这些变化进一步证明，睡眠不足可能对大脑健康产生不利影响，即使是在年轻人中。更大的队列有必要描述睡眠与昼夜节律机制，对长期复发性疾病的影响（例如，在轮班工人中），以及与其他生活方式和遗传因素的相互作用。
ObjectiveDisrupted sleep increases CSF levels of tau and beta -amyloid (A beta) and is associated with an increased risk of Alzheimer disease (AD). Our aim was to determine whether acute sleep loss alters diurnal profiles of plasma-based AD-associated biomarkers.MethodsIn a 2-condition crossover study, 15 healthy young men participated in 2 standardized sedentary in-laboratory conditions in randomized order: normal sleep vs overnight sleep loss. Plasma levels of total tau (t-tau), A beta 40, A beta 42, neurofilament light chain (NfL), and glial fibrillary acidic protein (GFAP) were assessed using ultrasensitive single molecule array assays or ELISAs, in the fasted state in the evening prior to, and in the morning after, each intervention.ResultsIn response to sleep loss (+17.2%), compared with normal sleep (+1.8%), the evening to morning ratio was increased for t-tau (p = 0.035). No changes between the sleep conditions were seen for levels of A beta 40, A beta 42, NfL, or GFAP (all p > 0.10). The AD risk genotype rs4420638 did not significantly interact with sleep loss-related diurnal changes in plasma levels of A beta 40 or A beta 42 (p > 0.10). Plasma levels of A beta 42 (-17.1%) and GFAP (-12.1%) exhibited an evening to morning decrease across conditions (p < 0.05).ConclusionsOur exploratory study suggests that acute sleep loss results in increased blood levels of t-tau. These changes provide further evidence that sleep loss may have detrimental effects on brain health even in younger individuals. Larger cohorts are warranted to delineate sleep vs circadian mechanisms, implications for long-term recurrent conditions (e.g., in shift workers), as well as interactions with other lifestyle and genetic factors.