The proteinase-activated receptor-2 mediates phagocytosis in a Rho-dependent manner in human keratinocytes.
The proteinase-activated receptor-2 mediates phagocytosis in a Rho-dependent manner in human keratinocytes.
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蛋白酶激活受体 2 在人角质形成细胞中以 Rho 依赖性方式介导吞噬作用。
DOI：
10.1046/j.1523-1747.2003.12427.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Han,Rujiing
中科院分区：
文献类型：
--
作者：
Scott,Glynis;Leopardi,Sonya;Parker,Lorelle;Babiarz,Laura;Seiberg,Miri;Han,Rujiing
Recent work shows that the G-protein-coupled receptor proteinase activated receptor-2 activates signals that stimulate melanosome uptake in keratinocytesin vivoandin vitro. The Rho family of GTP-binding proteins is involved in cytoskeletal remodeling during phagocytosis. We show that proteinase-activated receptor-2 mediated phagocytosis in human keratinocytes is Rho dependent and that proteinase-activated receptor-2 signals to activate Rho. In contrast, Rho activity did not affect either proteinase-activated receptor-2 activity or mRNA and protein levels. We explored the signaling mechanisms of proteinase-activated receptor-2 mediated Rho activation in human keratinocytes and show that activation of proteinase-activated receptor-2, either through specific proteinase-activated receptor-2 activating peptides or through trypsinization, elevates cAMP in keratinocytes. Proteinase-activated receptor-2 mediated Rho activation was pertussis toxin insensitive and independent of the protein kinase A signaling pathway. These data are the first to show that proteinase-activated receptor-2 mediated phagocytosis is Rho dependent and that proteinase-activated receptor-2 signals to Rho and cAMP in keratinocytes. Because phagocytosis of melanosomes is recognized as an important mechanism for melanosome transfer to keratinocytes, these results suggest that Rho is a critical signaling intermediate in melanosome uptake in keratinocytes.
