Lycopene attenuates alcoholic apoptosis in HepG2 cells expressing CYP2E1.
Lycopene attenuates alcoholic apoptosis in HepG2 cells expressing CYP2E1.
复制标题
番茄红素可减弱表达 CYP2E1 的 HepG2 细胞的酒精性细胞凋亡。
DOI：
10.1016/s0006-291x(03)01435-9
复制
发表时间：
2003
影响因子：
3.1
通讯作者：
Lieber,CharlesS
中科院分区：
文献类型：
--
作者：
Xu,Youqing;Leo,MariaA;Lieber,CharlesS
为了验证乙醇诱导的肝细胞凋亡是继发于细胞色素P4502E1（CYP2E1）产生的氧化应激的假设，我们评估了类胡萝卜素番茄红素（一种从番茄中提取的有效抗氧化剂）对过表达CYP2E1的HepG2细胞（2E1细胞）的氧化应激和凋亡的影响。将其暴露于100 mM乙醇和10μM番茄红素或等体积的安慰剂（溶媒）5天。流式细胞术和TUNEL法检测乙醇显著增加细胞凋亡。这是伴随着乙醇诱导的氧化应激：过氧化氢的生产显着增加，线粒体GSH显着下降。两者都被番茄红素恢复，细胞凋亡显著减少。安慰剂没有保护作用。总之，番茄红素对抗乙醇诱导的2E1细胞氧化应激和凋亡。这些效应之间的平行关系表明存在因果关系。此外，番茄红素的这些有益作用和无害性现在证明了体内试验的合理性。
To test the hypothesis that ethanol-induced hepatic apoptosis is secondary to the oxidative stress generated by cytochrome P4502E1 (CYP2E1), we assessed the effects of the carotenoid lycopene, a potent antioxidant extracted from tomatoes, on oxidative stress and apoptosis in HepG2 cells overexpressing CYP2E1 (2E1 cells). These were exposed for 5 days to 100mM ethanol and 10μM lycopene or an equal volume of placebo (vehicle). Ethanol significantly increased apoptosis measured by flow cytometry and by TUNEL assay. This was accompanied by an ethanol-induced oxidative stress: hydrogen peroxide production was significantly increased and mitochondrial GSH was strikingly decreased. Both were restored by lycopene, with a significant decrease in apoptosis. The placebo had no protective effect. In conclusion, Lycopene opposes the ethanol-induced oxidative stress and apoptosis in 2E1 cells. The parallelism between these effects suggests a causal link. Furthermore, these beneficial effects and the innocuity of lycopene now justify an in vivo trial.